In vitro bioactivity and gene expression by cells cultured on titanium dioxide doped phosphate-based glasses
In vitro bioactivity and gene expression by cells cultured on titanium dioxide doped phosphate-based glasses
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DOI：
10.1016/j.biomaterials.2007.03.018
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发表时间：
2007-07-01
期刊：
影响因子：
14
通讯作者：
Knowles, Jonathan Campbell
中科院分区：
文献类型：
--
作者：
Abou Neel, Ensanya Ali;Mizoguchi, Toshihide;Knowles, Jonathan Campbell
在我们以前的研究中，玻璃50 P_2O_5-（20-15）Na_2 O-30 CaO-（0-5 mol%）TiO_2已通过常规的熔体淬火工艺制备。本研究主要从MG 63细胞增殖、基因表达、生物相容性和生物活性等方面对玻璃进行了研究。结果表明，添加少量的TiO 2高达5摩尔%，增强了这些玻璃的生物相容性。细胞代谢活性是显著的，特别是在3和5mol%TiO 2组合物上，在21天的时间内与Thermanox对照没有显著差异。基因表达研究的结果表明，在第1天和5 mol% TiO 2玻璃上，核心结合蛋白因子α 1（Cbfa 1）和碱性磷酸酶（ALP）的转录水平显著降低;然而，I型胶原α亚基I（COLIAI）和骨连接素（Sparc）与对照组相比无显著差异。在第7天，所有这些基因的转录水平与对照组没有显著差异，但在第14天，它们显著高于对照组。此外，有没有在这些基因中检测到的3和5摩尔%的TiO 2玻璃长达7天的显着差异。然而，在第14天，5摩尔%的TiO 2玻璃显示出比3摩尔%的TiO 2组合物显著更高的水平。这也与5 mol% TiO 2组合物植入大鼠颅骨5周后骨-颗粒界面处新骨组织的存在相关。不管这种有利的细胞反应和基因上调，这些玻璃在SBF中孵育14天后没有显示出磷灰石层形成的证据。(C)2007爱思唯尔有限公司保留所有权利。
In our previous study, glasses with 50 P2O5-(20-15) Na2O-30 CaO-(0-5 mol%) TiO2 have been prepared by the conventional melt-quenching process. MG63 cell proliferation, gene expression, in vi vo biocompatibility, and bioactivity of these glasses is the concern of this study. The results showed that addition of TiO2 in small amounts up to 5 mol% enhanced the biocompatibility of these glasses. The cell metabolic activity was conspicuous, on 3 and 5mol% TiO2 compositions in particular, with no significant difference from Thermanox((R))) control over a period of 21 days. The findings from the gene expression study showed that, at day I and on 5 mol% TiO2 glass, core binding protein factor alpha 1 (Cbfa1) and alkaline phosphatase (ALP) showed significantly lower transcription level; however, collagen type I alpha subunit I (COLIAI) and Osteonectin (Sparc) showed no significant differences compared to the control. At day 7, all these genes transcription levels were not significantly different form the control, but at day 14, they were significantly higher than the control. Moreover, there were no significant differences detected in these genes on both 3 and 5 mol% TiO2 glasses up to 7 days. At day 14; however, 5 mol% TiO2 glasses showed significantly higher level than 3 mol% TiO2 composition. This was also correlated by the presence of new bone tissue at the bone-particles interface for 5 mol% TiO2 composition after 5 weeks of implantation in rat calvarium. Regardless of this favourable cell response and gene up-regulation, these glasses showed no evidence of apatite layer formation after 14 days incubation in SBF. (C) 2007 Elsevier Ltd. All rights reserved.