Expression of the IGFBP-2 gene in post-implantation rat embryos.
Expression of the IGFBP-2 gene in post-implantation rat embryos.
复制标题
DOI：
--
复制
发表时间：
1992
期刊：
影响因子：
4.6
通讯作者：
T. L. Wood;R. Streck;J. E. Pintar
中科院分区：
文献类型：
--
作者：
T. L. Wood;R. Streck;J. E. Pintar
胰岛素样生长因子（IGFs）在体内外刺激多种细胞类型的有丝分裂。这些作用由IGF受体和IGF结合蛋白（IGFBPs）家族介导，IGFBPs家族与血清和组织液中的IGF复合。在这里，我们比较的网站在早期大鼠胚胎发育的基因编码的含RGD的IGF结合蛋白IGFBP-2和IGF-II的表达。在从早期着床后到妊娠中期的所有年龄段，IGFBP-2的表达与IGF-II高度互补。IGFBP-2 mRNA的检测整个上胚层的卵圆筒早在E7，当IGF-II的表达仅限于滋养外胚层和其他胚外细胞。随着原肠胚形成的进行，IGFBP-2的表达停止，IGF-II的表达开始在新形成的胚胎和胚外中胚层，但保留在其他外胚层衍生物，包括表面外胚层和神经外胚层，在其整个吻尾的程度。通过e10-e11，IGFBP-2在神经外胚层中的表达被限制在初级神经管的嘴侧脑中，并且在次级神经胚形成期间在尾芽中形成的神经上皮的新群体中被发现。IGFBP-2的表达仍然很高，在脑室层的吻侧大脑到妊娠中期的年龄，但减少或消失的细胞进入外套层，并开始表达神经元相关基因α-internexin。IGFBP-2的mRNA是丰富的表面外胚层，特别是鳃弓，和所有的外胚层基板。（250字处删节）
The insulin-like growth factors (IGFs) stimulate mitogenesis in a variety of cell types both in vitro and in vivo. These effects are mediated by both IGF receptors and a family of IGF binding proteins (IGFBPs), which are found complexed with the IGFs in serum and tissue fluids. Here we compare the sites of expression during early rat embryogenesis of the genes encoding the RGD-containing IGF binding protein IGFBP-2 and IGF-II. At all ages from early post-implantation through mid-gestation, the expression of IGFBP-2 was highly complementary to IGF-II. IGFBP-2 mRNA was detected throughout the epiblast of the egg cylinder as early as e7, when IGF-II expression was restricted to trophectoderm and other extraembryonic cells. As gastrulation proceeded, IGFBP-2 expression ceased as IGF-II expression began in the newly formed embryonic and extra-embryonic mesoderm, but was retained in other epiblast derivatives including the surface ectoderm and neuroectoderm, throughout its rostral-caudal extent. By e10-e11, IGFBP-2 expression in neuroectoderm was restricted to the rostral brain of the primary neural tube and was found in the new population of neuroepithelium formed in the tail bud during secondary neurulation. IGFBP-2 expression remained high in the ventricular layer of the rostral brain into mid-gestation ages but decreased or disappeared as cells entered the mantle layer and began to express the neurofilament-related gene alpha-internexin. IGFBP-2 mRNA was abundant in surface ectoderm, particularly that of the branchial arches, and all ectodermal placodes.(ABSTRACT TRUNCATED AT 250 WORDS)