Heterogeneity of pituitary gonadotrope cells in male rats.
Heterogeneity of pituitary gonadotrope cells in male rats.
复制标题
雄性大鼠垂体促性腺细胞的异质性。
DOI：
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发表时间：
1997
影响因子：
--
通讯作者：
Dietrich Grube
中科院分区：
文献类型：
--
作者：
Thomas Jeziorowski;Thomas Jeziorowski;Tsuyoshi Watanabe;Tsuyoshi Watanabe;G. Bargsten;Dietrich Grube
哺乳动物促性腺激素（Gn-）细胞产生FSH和LH的细胞学特征表现出很大的变异性。为了更深入地了解相应的表型，我们采用免疫细胞化学、定量免疫细胞化学、三维重建、连续半薄/薄切片技术、常规电镜和免疫电镜等方法对雄性大鼠的Gn细胞进行了研究。用FSH和LH抗血清和针对嗜铬粒蛋白A（CgA）和分泌粒蛋白II（SgII）的合成N-和C-末端序列的抗体对Gn细胞进行免疫染色，可以鉴定出Gn细胞的四种表型。绝大多数Gn细胞对促性腺激素和嗜铬粒蛋白均呈密集的免疫反应性，因此被归类为"典型"促性腺激素;第二种类型对LH免疫反应性较低，因此被称为"LH-穷人"。其余两种表型显示超微结构，表明合成活性增加。其中一个被鉴定为"FSH/CgA-贫"促性腺激素。另一个，也在CGA穷人，形态学上对应的“印戒”细胞以前只知道在性腺切除动物。两种类型的分泌颗粒中存在的雄性大鼠Gn-细胞表现出严格的分离的嗜铬粒蛋白免疫反应：CGA仅限于大，不透明的颗粒，而SgII，小，电子致密颗粒。因此，这两个“CgA-穷人”的Gn细胞亚型表现出只有很少的大尺寸分泌颗粒。高细胞活性和减少在Gn细胞中CGA之间的相关性。Gn-细胞的形态和功能异质性的原因仍然是谜。据推测，它们要么对GnRH和睾酮的敏感性不同，要么受旁分泌因素的不同调节。
Mammalian gonadotrope (Gn-) cells producing FSH and LH show great variability in their cytological characteristics. To gain a closer view of the corresponding phenotypes, Gn-cells of male rats were investigated by various methods including immunocytochemistry on serial semithin sections, quantitative immunocytochemistry, three-dimensional reconstructions, sequential semithin-/thin-section technique, routine electron microscopy, and immuno-electron microscopy. Gn-cells were immunostained with FSH and LH antisera and with antibodies raised against synthetic N- and C-terminal sequences of chromogranin A (CgA) and secretogranin II (SgII), and four phenotypes of Gn-cells could be identified. The great majority of Gn-cells were densely immunoreactive for both gonadotropins and both chromogranins and accordingly classified as "typical" gonadotropes; a second type was less immunoreactive for LH and therefore was termed "LH-poor". The two remaining phenotypes showed ultrastructures suggesting an increased synthetic activity. One of them was characterized as a "FSH/CgA-poor" gonadotrope. The other, also poor in CgA, morphologically corresponded to "signet-ring" cells previously known only in gonadectomized animals. The two types of secretory granules present in male rat Gn-cells showed a strict segregation of chromogranin immunoreactivities: CgA was confined to the large, less opaque granules, whereas SgII, to the small, electron-dense granules. Consequently the two "CgA-poor" Gn-cell subtypes exhibited only a very few large-sized secretory granules. A correlation was suggested between high cellular activity and a decrease in CgA in Gn-cells. The reasons for the morphological and functional heterogeneity of Gn-cells remain enigmatic. Presumably, they either differ in the susceptibility for GnRH and testosterone or are differently regulated by paracrine factors.
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SIIp：垂体响应促性腺激素释放激素而释放的一种独特的促分泌素/嗜铬素。
影响因子：
4.8
作者：
Conn,PM;Janovick,JA;Braden,TD;Maurer,RA;Jennes,L
通讯作者：
Jennes,L
去势会诱导促卵泡激素β亚基信使核糖核酸的促性腺细胞群发生时间依赖性变化。
影响因子：
4.8
作者：
Childs,GV;Unabia,G;Wierman,ME;Gharib,SD;Chin,WW
通讯作者：
Chin,WW
大鼠垂体前叶表皮生长因子和转化生长因子-α信使核糖核酸及其受体：定位和调节。
DOI：
10.1210/endo.136.5.7720677
发表时间：
1995
期刊：
Endocrinology.
影响因子：
--
作者：
Fan,X;Childs,GV
通讯作者：
Childs,GV
发育中的去势细胞中促性腺激素储存的免疫细胞化学研究。
影响因子：
4.8
作者：
Childs,GV;Ellison,DG;Lorenzen,JR;Collins,TJ;Schwartz,NB
通讯作者：
Schwartz,NB
DOI：
10.1177/30.7.6286753
发表时间：
1982-07
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
--
作者：
G. Childs;G. Unabia
通讯作者：
G. Childs;G. Unabia