The effect of ultrasound at 1.5 MHz on Escherichia coli.
The effect of ultrasound at 1.5 MHz on Escherichia coli.
复制标题
1.5 MHz 超声波对大肠杆菌的影响。
DOI:
10.1080/09553008014550131
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发表时间:
1980
期刊:
影响因子:
--
通讯作者:
W. Cramp
中科院分区:
文献类型:
--
作者:
M. Hedges;M. Lewis;J. Lunec;W. Cramp
2. Materials and methods The bacteria were grown and their DNA labelled with 3 H-thymidine in minimal medium as described by Cramp and Watkins (1970). The labelling was for one and a half divisions followed by a chase in fresh medium with cold thymidine over a quarter of a division. The cells were harvested by centrifugation and finally resuspended in 0. 15 M sodium chloride. The cells were cooled to ice temperature before ultrasound irradiation (insonation).