MAGOH interacts with a novel RNA-binding protein
MAGOH interacts with a novel RNA-binding protein
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DOI：
10.1006/geno.1999.6064
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发表时间：
2000-01-01
期刊：
影响因子：
4.4
通讯作者：
Aplan, PD
中科院分区：
文献类型：
--
作者：
Zhao, XF;Nowak, NJ;Aplan, PD
MAGOH 是果蝇 mago nashi 的人类同源物，果蝇胚胎中正常种质发育所需的蛋白质。使用人类 MAGOH 作为酵母双杂交筛选中的诱饵蛋白，我们恢复了四个独立的 cDNA 克隆，它们编码不同长度的包含保守 RNA 结合区域的新蛋白质。该基因被命名为 RBM8，编码 173 个氨基酸的蛋白质，体外翻译测定表明该蛋白质的表观分子质量为 26 kDa。 MAGOH 和 RBM8 之间的相互作用通过酵母双杂交和 GST 融合蛋白下拉测定得到证明。与 MAGOH 一样，RBM8 基因在人体组织中普遍表达；检测到三种RBM8 mRNA。与 MAGOH 类似，RBM8 表达在静止的 NIH3T3 成纤维细胞中可通过血清诱导。 (C) 2000 年学术出版社。
MAGOH is the human homologue of Drosophila mago nashi, a protein that is required for normal germ plasm development in the Drosophila embryo, Using human MAGOH as a bait protein in a yeast two-hybrid screen, we recovered four independent cDNA clones that encode different lengths of a novel protein containing a conserved RNA-binding region. This gene, designated RBM8, encodes a 173-aa protein that was shown to have an apparent molecular mass of 26 kDa, as demonstrated by in vitro translation assay. The interaction between MAGOH and RBM8 was demonstrated by both yeast two-hybrid and GST fusion protein pull-down assays. Like MAGOH, RBM8 gene is expressed ubiquitously in human tissues; three species of RBM8 mRNA were detected. Also similar to MAGOH, RBM8 expression is serum inducible in quiescent NIH3T3 fibroblast cells. (C) 2000 Academic Press.