The Flavin-Containing Monooxygenase 3 Gene and Essential Hypertension: The Joint Effect of Polymorphism E158K and Cigarette Smoking on Disease Susceptibility.
The Flavin-Containing Monooxygenase 3 Gene and Essential Hypertension: The Joint Effect of Polymorphism E158K and Cigarette Smoking on Disease Susceptibility.
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DOI：
10.1155/2014/712169
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发表时间：
2014
影响因子：
1.9
通讯作者：
Polonikov A
中科院分区：
文献类型：
--
作者：
Bushueva O;Solodilova M;Churnosov M;Ivanov V;Polonikov A
含有黄素的单加氧酶3（FMO 3）基因是一种微粒体抗氧化防御酶，被认为与原发性高血压（EH）有关。本研究的目的是调查是否常见的功能多态性E158 K（rs 2266782）的FMO 3基因与高血压的易感性在俄罗斯人口。本研究共招募了来自俄罗斯中部库尔斯克（1 362例EH患者和843例健康对照）和别尔哥罗德（357例EH患者和422例人群对照）地区的2 995例无关受试者。所有研究参与者的DNA样本通过PCR进行FMO 3基因多态性基因分型，然后进行RFLP分析。经性别和年龄校正后，我们发现E158 K多态性与库尔斯克地区发现人群（OR 1.36 95% CI 1.09-1.69，P = 0.01）和别尔哥罗德地区复制人群（OR 1.54 95% CI 1.07-1.89，P = 0.02）原发性高血压的风险增加有关。进一步分析表明，158 KK基因型携带者的高血压风险增加发生在吸烟者中，而非吸烟者则无此风险。这是第一个报道FMO 3基因多态性与原发性高血压风险相关的研究。
Gene encoding flavin-containing monooxygenase 3 (FMO3), a microsomal antioxidant defense enzyme, has been suggested to contribute to essential hypertension (EH). The present study was designed to investigate whether common functional polymorphism E158K (rs2266782) of the FMO3 gene is associated with EH susceptibility in a Russian population. A total of 2 995 unrelated subjects from Kursk (1 362 EH patients and 843 healthy controls) and Belgorod (357 EH patients and 422 population controls) regions of Central Russia were recruited for this study. DNA samples from all study participants were genotyped for the FMO3 gene polymorphism through PCR followed by RFLP analysis. We found that the polymorphism E158K is associated with increased risk of essential hypertension in both discovery population from Kursk region (OR 1.36 95% CI 1.09–1.69, P = 0.01) and replication population from Belgorod region (OR 1.54 95% CI 1.07–1.89, P = 0.02) after adjustment for gender and age using logistic regression analysis. Further analysis showed that the increased hypertension risk in carriers of genotype 158KK gene occurred in cigarette smokers, whereas nonsmoker carriers of this genotype did not show the disease risk. This is the first study reporting the association of the FMO3 gene polymorphism and the risk of essential hypertension.