Completion of cytokinesis in C-elegans requires a brefeldin A-sensitive membrane accumulation at the cleavage furrow apex
Completion of cytokinesis in C-elegans requires a brefeldin A-sensitive membrane accumulation at the cleavage furrow apex
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DOI：
10.1016/s0960-9822(01)00231-7
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发表时间：
2001-05-15
期刊：
影响因子：
9.2
通讯作者：
White, JG
中科院分区：
文献类型：
--
作者：
Skop, AR;Bergmann, D;White, JG
背景资料：真核细胞胞质分裂的终末阶段涉及包含纺锤体中间区的细胞间通道的断裂和子细胞的重新密封。最近的观察表明，纺锤体中间区是需要这个过程。在这项研究中，我们研究了纺锤体中间区附近的靶向分泌是胞质分裂终末期执行所必需的可能性。使用分裂细胞的4D记录，我们表明BFA在胞质分裂的终末阶段诱导定型失败;虽然沟正常进入，但几分钟后沟完全消退，即使纺锤体中区和中间体微管出现正常。此外，使用FM 1 -43膜探针，我们发现，膜局部积累在后期卵裂沟的顶点，形成持续的子细胞之间的细胞间管道。然而，在BFA处理的胚胎中，这种膜积累没有发生，这可能是观察到的卵裂failure.Conclusions：我们已经表明，BFA破坏了在C胚胎卵裂球胞质分裂的终末阶段。优雅的我们观察到，膜积累在尖端的后期卵裂沟的BFA敏感的机制。我们认为，这种局部膜积累对于胞质分裂的完成是必要的，并推测动物细胞的纺锤体中区区域在功能上与植物的成膜体相当，并且起到将分泌物靶向至分裂细胞赤道面的作用。
Background: The terminal phase of cytokinesis in eukaryotic cells involves breakage of the intercellular canal containing the spindle midzone and resealing of the daughter cells. Recent observations suggest that the spindle midzone is required for this process. In this study, we investigated the possibility that targeted secretion in the vicinity of the spindle midzone is required for the execution of the terminal phase of cytokinesis,Results: We inhibited secretion in early C. elegans embryos by treatment with brefeldin A (BFA), Using 4D recordings of dividing cells, we showed that BFA induced stereotyped failures in the terminal phase of cytokinesis; although the furrow ingressed normally, after a few minutes the furrow completely regressed, even though spindle midzone and midbody microtubules appeared normal. In addition, using an FM1-43 membrane probe, we found that membrane accumulated locally at the apices of the late cleavage furrows that form the persisting intercellular canals between daughter cells. However, in BFA-treated embryos this membrane accumulation did not occur, which possibly accounts for the observed cleavage failures.Conclusions: We have shown that BFA disrupts the terminal phase of cytokinesis in the embryonic blastomeres of C. elegans. We observed that membrane accumulates at the apices of the late cleavage furrow by means of a BFA-sensitive mechanism. We suggest that this local membrane accumulation is necessary for the completion of cytokinesis and speculate that the spindle midzone region of animal cells is functionally equivalent to the phragmoplast of plants and acts to target secretion to the equatorial plane of a cleaving cell.