Dynamics of sonoporation correlated with acoustic cavitation activities.
Dynamics of sonoporation correlated with acoustic cavitation activities.
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DOI：
10.1529/biophysj.107.125617
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发表时间：
2008-04
影响因子：
3.4
通讯作者：
Yun Zhou;J. Cui;C. Deng
中科院分区：
文献类型：
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作者：
Yun Zhou;J. Cui;C. Deng
声纳效应已被开发为一种有前途的非病毒策略，用于细胞内递送药物和基因。该技术利用超声应用，通常由微泡的存在促进，在细胞膜上产生瞬时的非特异性孔。然而，由于超声介导的气泡与细胞相互作用的复杂性和瞬态性，声波穿孔与气泡活动（如声空化，即超声驱动的气泡生长和剧烈崩溃）没有直接关联。本研究以非洲爪蟾卵母细胞为模型系统，实时研究了局部空化对单个细胞声穿孔的影响。采用共聚焦共直对准双频超声换能器组件，产生聚焦超声脉冲（1.5 MHz），诱导焦点声谐振，同时检测同一焦点区域内的宽带空化声发射。在电压箝位下，通过细胞的跨膜电流监测单个细胞的动态声穿孔。据我们所知，我们的结果首次在单细胞水平上证明了声谐振与空化的时空相关性。
Sonoporation has been exploited as a promising nonviral strategy for intracellular delivery of drugs and genes. The technique utilizes ultrasound application, often facilitated by the presence of microbubbles, to generate transient, nonspecific pores on the cell membrane. However, due to the complexity and transient nature of ultrasound-mediated bubble interaction with cells, no direct correlation of sonoporation with bubble activities such as acoustic cavitation, i.e., the ultrasound-driven growth and violent collapse of bubbles, has been obtained. Using Xenopus oocytes as a model system, this study investigated sonoporation in a single cell affected by colocalized cavitation in real time. A confocally and collinearly-aligned dual-frequency ultrasound transducer assembly was used to generate focused ultrasound pulses (1.5 MHz) to induce focal sonoporation while detecting the broadband cavitation acoustic emission within the same focal zone. Dynamic sonoporation of the single cell was monitored via the transmembrane current of the cell under voltage-clamp. Our results demonstrate for the first time, to our knowledge, the spatiotemporal correlation of sonoporation with cavitation at the single-cell level.