Inhibition of Stat3 signaling pathway decreases TNF--induced autophagy in cementoblasts
Inhibition of Stat3 signaling pathway decreases TNF--induced autophagy in cementoblasts
复制标题
抑制 Stat3 信号通路可减少 TNF-α 诱导的成牙骨质细胞自噬。
DOI：
10.1007/s00441-018-2890-2
复制
发表时间：
2018-12-01
影响因子：
3.6
通讯作者：
He, Hong
中科院分区：
文献类型：
--
作者：
Wang, Leilei;Wang, Yunlong;He, Hong
Autophagy is a self-digestive process that eliminates impaired or aged proteins and potentially toxic intracellular components to maintain homeostasis. We previously demonstrated that TNF- played a critical role in cementoblast differentiation, mineralization and apoptosis; however, the effect of TNF- on cementoblast autophagy has remained unclear. In this study, an elevated immunofluorescence signal of LC3B and autophagic vacuoles, autophagosomes and autolysosomes were detected under TNF- stimulation in OCCM-30 cells. Autophagy-related genes and proteins, Beclin-1, LC3A and Atg-5, were significantly upregulated by TNF- in a time- and concentration-dependent manner. During this process, the activity of Stat3 was dramatically enhanced and when the activity of Stat3 was blocked by either a specific chemical inhibitor or siRNA transfection before TNF- stimulation, the TNF--induced upregulation of autophagy-related genes and proteins was strongly inhibited. Our results suggest that TNF- induced autophagy in cementoblasts was dependent, or partially dependent on the activity of Stat3 signaling pathway.
