Expression of the hyaluronan receptor RHAMM in endometrial carcinomas suggests a role in tumour progression and metastasis
Expression of the hyaluronan receptor RHAMM in endometrial carcinomas suggests a role in tumour progression and metastasis
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DOI：
10.1007/s00432-003-0415-0
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发表时间：
2003-03-01
影响因子：
3.6
通讯作者：
Dall, P
中科院分区：
文献类型：
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作者：
Rein, DT;Roehrig, K;Dall, P
目的：透明质酸（HA）与其结合蛋白RHAMM （HA介导的运动受体）的相互作用被认为是促进肿瘤进展和传播的重要因素。本研究旨在探讨子宫内膜癌中RHAMM的表达模式。方法：应用rhamm特异性多克隆抗体对89例子宫内膜癌和15例正常子宫内膜组织进行免疫组化检测。根据肿瘤内（整个细胞质、肿瘤中心/周围）和肿瘤间的模式和强度评估RHAMM的表达。将染色结果与相应的临床资料（年龄、绝经状况、组织学染色、组织学分级、淋巴结状况）进行比较。结果：89例肿瘤中有58%检测到rhamm表达[组织学分期：pT1a (8/12): pT1b (16/37)；pT1c (18/26);pT2 (6/9);pT3a(4/5)]和13%（2/15）的正常子宫内膜组织。淋巴结阳性患者RHAMM阳性率为100%，而淋巴结阴性患者阳性率仅为50.7% (P
Purpose: Interactions of hyaluronic acid (HA) with its binding protein RHAMM (receptor for HA-mediated motility) have been proposed as being important in promoting tumour progression and dissemination. This comparative study was designed to investigate the RHAMM expression patterns in endometrial carcinoma. Methods: We examined a series of 89 endometrial carcinomas and 15 normal endometrial tissues by immunohistochemistry, using a RHAMM-specific polyclonal antibody. Expression of RHAMM was assessed according to the pattern and intensity within (overall cytoplasm, center/periphery of tumours) and between the tumours. The staining results were compared to the corresponding clinical data (age, menopause status, histological staining, histological grading, lymph node status). Results: RHAMM-expression was detectable in 58% of the 89 tumours [Histological stage: pT1a (8/12): pT1b (16/37); pT1c (18/26); pT2 (6/9); pT3a (4/5)] and 13% (2/15) of the normal endometrial tissues. The positivity rates for RHAMM were 100% in patients with positive lymph nodes but only 50.7% in patients with negative lymph nodes (P