Biological approaches to artificial photosynthesis, fundamental processes and theoretical approaches: general discussion.
Biological approaches to artificial photosynthesis, fundamental processes and theoretical approaches: general discussion.
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人工光合作用的生物学方法、基本过程和理论方法：一般性讨论。
DOI：
10.1039/c7fd90016c
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发表时间：
2017
影响因子：
3.4
通讯作者：
Artero V
中科院分区：
文献类型：
--
作者：
Artero V
井上春男主持了岛清吾论文的讨论：谢谢你的精彩演讲。CO2转化为甲烷的过程需要8个步骤，氢的电子以有机氢化物的形式转化一次。对于化学家来说，想象氢化物在哪里以及如何与CO2相互作用的分子机制以及在它们相互作用之前CO2是如何被捕获的有点困难。你能解释一下这些观点吗？岛清吾回答说：在氢营养型产甲烷途径中，CO2被铁氧还蛋白的两个电子还原。该反应由甲酰甲烷呋喃脱氢酶（Fwd）催化。该酶系统中的CO2还原由与含Mo或W的甲酸脱氢酶（Fdh）同源的亚基催化。根据提出的Fdhs的催化机制，在Fwd中，一个氢化物或两个电子似乎从钨络合物转移到活化形式的CO2，其被捕获在金属位点或位于金属位点附近的精氨酸侧链上。
Haruo Inoue opened the discussion of the paper by Seigo Shima: Thank you for your nice talk. The conversion process of CO2 into methane takes 8 steps and the electrons of hydrogen are once converted in the form of organic hydride. It is a bit difficult for a chemist to imagine the molecular mechanism where and how the hydride interacts with CO2 and how CO2 is captured prior to their interaction. Could you explain about those points?Seigo Shima answered: In the hydrogenotrophic methanogenic pathway, CO2 is reduced with two electrons from ferredoxin. This reaction is catalyzed by formylmethanofuran dehydrogenase (Fwd). CO2 reduction in this enzyme system is catalyzed by the subunits that are homologous to Mo-or W-containing formate dehydrogenase (Fdh). According to the proposed catalytic mechanisms of Fdhs, In Fwd, a hydride, or two electrons, appears to transfer from the tungsten complex to the activated form of CO2, which was captured at the metal site or at the side chain of arginine located near the metal site.