Regulation of stomatal opening and histone modification by photoperiod in Arabidopsis thaliana
Regulation of stomatal opening and histone modification by photoperiod in Arabidopsis thaliana
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DOI：
10.1038/s41598-019-46440-0
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发表时间：
2019-07-22
影响因子：
4.6
通讯作者：
Kinoshita, Toshinori
中科院分区：
文献类型：
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作者：
Aoki, Saya;Toh, Shigeo;Kinoshita, Toshinori
气孔运动受许多环境信号的调节，如光、CO2、温度、湿度和干旱。最近，我们发现光周期开花成分对光诱导的气孔开放有积极的影响。在这项研究中，我们确定，光诱导的气孔开放和气孔导度增加长日照（LD）条件下生长的植物比短日照（SD）条件下生长的植物更大。使用纯化的保卫细胞原生质体的基因表达分析显示，FT和SOC 1的表达水平显着增加LD条件下。有趣的是，增强光诱导的气孔开放和增加SOC 1的表达在保卫细胞由于LD条件下持续至少1周后，植物转移到SD条件。然后，我们研究了组蛋白修饰染色质免疫沉淀-PCR，并观察到增加的三甲基化组蛋白3（H3 K4）周围SOC 1的赖氨酸4。我们还发现，LD依赖的增强光诱导的气孔开放和H3 K4三甲基化的SOC 1被抑制盗窃-2突变体。这些结果表明，光周期是一个重要的环境线索调节气孔开放，和LD条件下提高光诱导的气孔开放和表观遗传修饰（H3 K4三甲基化）周围SOC 1，气孔开放的正调节，在FT依赖的方式。因此，这项研究提供了新的见解气孔对光周期的反应。
Stomatal movements are regulated by many environmental signals, such as light, CO2, temperature, humidity, and drought. Recently, we showed that photoperiodic flowering components have positive effects on light-induced stomatal opening in Arabidopsis thaliana. In this study, we determined that light-induced stomatal opening and increased stomatal conductance were larger in plants grown under long-day (LD) conditions than in those grown under short-day (SD) conditions. Gene expression analyses using purified guard cell protoplasts revealed that FT and SOC1 expression levels were significantly increased under LD conditions. Interestingly, the enhancement of light-induced stomatal opening and increased SOC1 expression in guard cells due to LD conditions persisted for at least 1 week after plants were transferred to SD conditions. We then investigated histone modification using chromatin immunoprecipitation-PCR, and observed increased trimethylation of lysine 4 on histone 3 (H3K4) around SOC1. We also found that LD-dependent enhancement of light-induced stomatal opening and H3K4 trimethylation in SOC1 were suppressed in theft-2 mutant. These results indicate that photoperiod is an important environmental cue regulating stomatal opening, and that LD conditions enhance light-induced stomatal opening and epigenetic modification (H3K4 trimethylation) around SOC1, a positive regulator of stomatal opening, in an FT-dependent manner. Thus, this study provides novel insights into stomatal responses to photoperiod.