Renal expression of monocyte chemotactic protein-1 and epidermal growth factor in children with obstructive hydronephrosis
Renal expression of monocyte chemotactic protein-1 and epidermal growth factor in children with obstructive hydronephrosis
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DOI：
10.1053/jpsu.2000.0350569
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发表时间：
2000-04-01
影响因子：
2.4
通讯作者：
Leggio, A
中科院分区：
文献类型：
--
作者：
Bartoli, F;Gesualdo, L;Leggio, A
背景/目的：通过检测肾盂输尿管连接部梗阻（UPJ O）患儿肾脏表皮生长因子（EGF）和单核细胞趋化蛋白-1（MCP-1）mRNA的表达，探讨UPJ O在儿童进行性肾损害中的作用。取11例UPJ-O患儿和10例正常儿童肾组织，研究EGF和MCP-1在UPJ-O患儿肾组织中的表达。1通过原位杂交检测。结果：UPJ-O患儿表皮生长因子（EGF）基因表达明显低于对照组。MCP-1 mRNA在UPJ-0病例间质中均有表达。EGF和MCP-1的表达与年龄、肾功能分级和通过MAG 3肾扫描测量的肾厚度无关。与无UTI病史的儿童相比，有UTI病史的儿童受影响肾脏的肾脏厚度减少更严重。MCP-1的表达更高，EGF更减少，在儿童的历史UTI.Conclusions：我们的研究结果表明，潜在的作用，EGF和MCP-1的肾损害和生长衰竭UPJ-O的发病机制，特别是在儿童与UTI。这些重要的功能变化开始在生命早期，可能在胎儿期。Copyright（C）2000 by W.B.桑德斯公司
Background/Purpose: The authors studied the potential role of ureteropelvic junction obstruction (UPJ-O) in causing progressive renal damage in children through the renal expression of epidermal growth factor (EGF) and monocyte chemotactic protein-1 (MCP-1) mRNA.Methods: Renal tissues were harvested from 11 children with UPJ-O and from 10 normal kidneys to study the renal expression of EGF and MCP-1 detected by means of in situ hybridization. Five of the patients were found to have a history of urinary tract infection (UTI),Results: Children with UPJ-O had marked reduction of EGF gene expression when compared with controls. Interstitial expression of MCP-1 mRNA was present in all UPJ-O cases. Both EGF and MCP-1 expression did not correlate with age, with differential renal function, and with renal thickness measured through MAG3 renal scan. Children with a history of UTI had a more severe reduction of the renal thickness of the affected kidney compared with those without UTI. MCP-1 expression was higher and EGF more reduced in children with a history of UTI.Conclusions: Our results suggest a potential role of EGF and MCP-1 in the pathogenesis of renal damage and growth failure in UPJ-O, especially in children with UTI. These important functional changes begin early in life, possibly during fetal life. Copyright (C) 2000 by W.B. Saunders Company.