Vectored antibody gene delivery mediates long-term contraception.
Vectored antibody gene delivery mediates long-term contraception.
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载体抗体基因递送介导长期避孕。
DOI：
10.1016/j.cub.2015.08.002
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
Hay,BruceA
中科院分区：
文献类型：
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作者：
Li,Juan;Olvera,AlejandraI;Akbari,OmarS;Moradian,Annie;Sweredoski,MichaelJ;Hess,Sonja;Hay,BruceA
开发长期或永久避孕的非手术方法仍然是一项挑战。为了实现这一目标，我们表明，肌肉注射的复制能力，重组腺相关病毒（rAAV）的目的是表达一种抗体，结合促性腺激素释放激素（GnRH），在脊椎动物中的主要调节生殖，结果在长期不育的男性和女性小鼠。雌性小鼠也通过rAAV依赖性表达结合透明质酸（ZP）的抗体而变得不育，透明质酸（ZP）是一种围绕卵子并作为精子结合位点的糖蛋白基质。许多已知或怀疑对生殖重要的蛋白质可以使用这种方法进行靶向，可能是可逆的，我们称之为载体避孕（VC）。
Development of non-surgical methods of long-term or permanent contraception remains a challenge. Towards this objective, we show that intramuscular injection of a replication-incompetent, recombinant adeno-associated virus (rAAV) designed to express an antibody that binds gonadotropin-releasing hormone (GnRH), a master regulator of reproduction in vertebrates, results in long-term infertility in male and female mice. Female mice are also rendered infertile through rAAV-dependent expression of an antibody that binds to the zona pellucida (ZP), a glycoprotein matrix that surrounds the egg and functions as a sperm-binding site. Many proteins known or suspected to be important for reproduction can be targeted, potentially reversibly, using this approach, which we refer to as vectored contraception (VC).