Lung epithelial cells and type II pneumocytes of donor origin after allogeneic hematopoietic stem cell transplantation
Lung epithelial cells and type II pneumocytes of donor origin after allogeneic hematopoietic stem cell transplantation
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DOI：
10.1097/01.tp.0000132326.08628.74
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发表时间：
2004-07-15
期刊：
影响因子：
6.2
通讯作者：
Hassan, M
中科院分区：
文献类型：
--
作者：
Mattson, J;Jansson, M;Hassan, M
背景在本研究中，我们确定是否供者上皮肺细胞可能会检测异基因造血干细胞移植（HSCT）后。在非清髓性HSCT后，从四名女性患者尸检时获得肺组织标本，其中两名为男性供体。细胞角蛋白免疫组化染色用于鉴定肺上皮细胞。采用荧光原位杂交技术检测供者肺上皮细胞的存在情况。所有患者在HSCT后的所有细胞系中均表现出几乎完全的供体嵌合体。在两个阳性对照中，在每个切片中检测到2%至6%的Y染色体阳性肺上皮细胞。还检测到表面活性剂阳性的男性上皮细胞，表明II型肺细胞的植入。在两个阴性对照中，未检测到Y染色体阳性细胞。循环供体干细胞在异基因HSCT后可分化为肺上皮细胞。
Background. In the present investigation, we determined whether donor epithelial lung cells might be detected after allogeneic hematopoietic stem cell transplantation (HSCT).Methods. Lung-tissue specimens were obtained at autopsy from four female patients, two with male donors, after nonmyeloablative HSCT. Immunohistochemical staining for cytokeratin was used to identify lung epithelial cells. The tissue sections were analyzed for the presence of donor-derived lung epithelial cells with the use of fluorescence in situ hybridization of XY-positive cells.Results. All patients showed almost complete donor chimerism in all cell lineages after HSCT with polymerase chain reaction of minisatellites. In the two positive controls, between 2% and 6% Y-chromosome-positive epithelial lung cells were detected in each section. Surfactant-positive male epithelial cells were also detected, indicating engraftment of type II pneumocytes. In the two negative controls, no Y-chromosome-positive cells were detected.Conclusion. Circulating donor stem cells may differentiate into lung epithelial cells after allogeneic HSCT.