Effect of temperature on sulfonamide antibiotics degradation, and on antibiotic resistance determinants and hosts in animal manures
Effect of temperature on sulfonamide antibiotics degradation, and on antibiotic resistance determinants and hosts in animal manures
复制标题
温度对磺酰胺类抗生素降解以及动物粪便中抗生素耐药性决定因素和宿主的影响
DOI：
10.1016/j.scitotenv.2017.07.057
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发表时间：
2017-12-31
影响因子：
9.8
通讯作者：
Ma, Junwei
中科院分区：
文献类型：
--
作者：
Lin, Hui;Zhang, Jin;Ma, Junwei
动物粪便是抗生素残留和抗生素耐药性的主要储存库。本文通过在不同温度下对猪粪和鸡粪进行好氧培养，研究了温度对磺胺类抗生素（SA）、磺胺类耐药（SR）基因/细菌的影响。在猪粪中，SA浓度随温度升高而降低，在60 ℃时最低。在鸡粪中，SA的最大降解是在30摄氏度下。抗生素抗性基因（ARGs）和硫阳性主机在猪粪中的相对丰度的减少是更明显的嗜热比中层孵育期间，既没有温度条件有效地降低了这些参数在鸡粪。进一步建立了SA、ARG（sul 1、sul 2和intI 1）、可培养SR细菌和sul-positive宿主的残留水平/分布曲线之间的关系。抗生素残留概况，而不是抗生素浓度，作为一个重要因素的流行ARG和硫阳性主机的粪肥。来自放线菌门的棒状杆菌和亮杆菌倾向于成为sul 1和intI 1的主要载体; sul 2的相对丰度与可培养SR细菌的相对丰度显著相关。总体而言，耐药细菌群落的差异也构成了影响ARG变异的主导因素。这项研究有助于减少粪便中抗生素污染和抗生素耐药性的管理选择。(C)2017爱思唯尔B. V.保留所有权利。
Animal manure is a main reservoir of antibiotic residues and antibiotic resistance. Here, the effect of temperature on sulfonamide antibiotics (SAs), sulfonamide-resistant (SR) genes/bacteria was investigated by aerobically incubating swine and chicken manures at different temperatures. In swine manure, the SAs concentration declined with increasing temperature, with a minimum at 60 degrees C. In chicken manure, the greatest degradation of SAs was noted at 30 degrees C. The reduction of relative abundance of antibiotic resistance genes (ARGs) and sul-positive hosts in swine manure was more pronounced during thermophilic than mesospheric incubation; neither temperature conditions effectively reduced these parameters in chicken manure. The relationship between the residual levels/distribution profiles of SAs, ARGs (sul1, sul2 and intI1), cultivable SR bacteria and sul-positive hosts was further established. The antibiotic residual profile, rather than antibiotic concentration, acted as an important factor in the prevalence of ARGs and sul-positive hosts in manure. Corynebacterium and Leucobacter from the phylum Actinobacteria tend to be main carriers of sul1 and intI1; the relative abundance of sul2 was significantly correlated with the relative abundance of cultivable SR bacteria. Overall, differences in resistant bacterial communities also constitute a dominant factor affecting ARG variation. This study contributes to management options for reducing the pollution of antibiotics and antibiotic resistance within manure. (C) 2017 Elsevier B.V. All rights reserved.