Antioxidant performance in droplet-stabilized oil-in-water emulsions
Antioxidant performance in droplet-stabilized oil-in-water emulsions
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DOI：
10.1016/j.lwt.2020.110541
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发表时间：
2021-01-27
影响因子：
6
通讯作者：
Loveday, Simon M.
中科院分区：
文献类型：
--
作者：
Okubanjo, Sewuese S.;Ye, Aiqian;Loveday, Simon M.
液滴稳定乳剂（DSE）由较小的蛋白质包被的脂滴（外壳）稳定的大脂滴（核心）组成，这种结构有效地保护不饱和脂质免受氧化。本研究调查和比较了抗氧化剂（丁基羟基茴香醚- BHA）的性能时，无论是在壳液滴或核心脂质的DSE。乳液由多不饱和脂肪酸（PUFA）油核心与壳乳液乳化组成，壳乳液含有低（橄榄油）或高（三肉豆蔻酸）熔点脂质。在亚铁（500 μ mol/L）存在下，用荧光灯加速乳剂的氧化11天，并通过形成共轭二烯，脂质过氧化氢和己醛监测PUFA氧化。在500 mg/kg BHA下，在具有壳中BHA液滴的DSE中的氧化比具有核中BHA PUFA油的乳液中的氧化慢，但在50 mg/kg BHA下观察到相反的趋势。在低于壳脂质熔融温度下加工的三肉豆蔻酸甘油酯DSE在两种BHA水平下均具有最大的抗氧化性。结果表明，将BHA加入DSEs的壳液滴中可能比将其加入核心PUFA油中更有效，并且DSEs中的BHA性能可能取决于其浓度、转移机制和与反应位点的接近程度。
Droplet-stabilized emulsions (DSEs) consist of large lipid droplets (the core) stabilized by smaller, protein-coated lipid droplets (the shell) and this structure effectively protects unsaturated lipids from oxidation. This study investigated and compared performance of an antioxidant (butylated hydroxyanisole- BHA) when incorporated either in shell droplets or in the core lipid of DSEs. Emulsions consisted of a core of polyunsaturated fatty acid (PUFA) oil emulsified with shell emulsions that contained either low (olive oil), or high (trimyristin) melting point lipids. Oxidation of emulsions was accelerated with a fluorescent lamp in the presence of ferrous iron (500 mu mol/L) for eleven days, and PUFA oxidation was monitored via the formation of conjugated dienes, lipid hydroperoxides and hexanal. At 500 mg/kg BHA, oxidation was slower in DSEs with BHA-in-shell droplets than in emulsions with BHA-in-core PUFA oil, but the reverse trend was observed at 50 mg/kg BHA. Trimyristin DSEs processed below the shell lipid melting temperature gave the greatest oxidation resistance at both BHA levels. Results suggest that incorporating BHA into shell droplets of DSEs can be more effective than incorporating it into core PUFA oil, and BHA performance in DSEs may be dependent on its concentration, transfer mechanism and proximity to reaction sites.