Identification of spider mites (Acari: Tetranychidae) by DNA sequences:: A case study in Northern Vietnam
Identification of spider mites (Acari: Tetranychidae) by DNA sequences:: A case study in Northern Vietnam
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DOI：
10.1080/01647950708684501
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发表时间：
2007-03-01
影响因子：
1.1
通讯作者：
Takafuji, Akio
中科院分区：
文献类型：
--
作者：
Hinomoto, Norihide;Tran, Dinh Pha;Takafuji, Akio
叶螨的种类鉴定一直是害虫防治中的一个重要而复杂的问题。我们研究了在越南北方农业领域的蜘蛛螨的物种组成，通过使用线粒体细胞色素氧化酶亚基I序列作为一个案例研究的DNA条形码识别技术的蜘蛛螨。通过将获得的DNA序列与DNA数据库中保存的已知序列进行比较来鉴定标本。如果将系统发育分析与DNA条形码技术结合起来，则DNA条形码技术可用于叶螨的种间鉴定，特别是叶螨属的种间鉴定。利用DNA条形码技术，我们将大部分越南样品分为神泽叶螨、二斑叶螨和截形叶螨。
Identification of spider mite species has been an important but complicated issue in pest control. We studied the species composition of spider mites collected in agricultural fields of northern Vietnam by using mitochondrial cytochrome oxidase subunit I sequences as a case study of the DNA barcoding identification technique for spider mites. Specimens were identified by comparing obtained DNA sequences with known sequences deposited in DNA databases. If phylogenetic analysis was used together, the DNA barcoding approach was useful for species identification of spider mites, especially for the genus Tetranychus. Using DNA barcoding, we classified most of the Vietnamese samples into Tetranychus kanzawai Kisbida, T urticae Koch and T truncatus Ehara.