Disruption of the Pfg27 locus by homologous recombination leads to loss of the sexual phenotype in P-falciparum
Disruption of the Pfg27 locus by homologous recombination leads to loss of the sexual phenotype in P-falciparum
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DOI：
10.1016/s1097-2765(01)80011-3
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发表时间：
1999-06-01
期刊：
影响因子：
16
通讯作者：
Kumar, N
中科院分区：
文献类型：
--
作者：
Lobo, CA;Fujioka, H;Kumar, N
疟疾的传播取决于寄生虫性阶段的分化和发育。在恶性疟原虫中，这是一个复杂的多阶段过程，涉及大量性阶段特异性蛋白的表达。Pfg27就是这样一种蛋白质，在配子体发生开始时大量表达。我们报告了使用同源重组成功破坏Pfg27位点，并表明它对维持性表型至关重要。缺乏Pfg27的转染子在性发育早期流产，导致空泡化、高度无序和崩解的寄生虫。这表明Pfg27在寄生虫的性发育中起关键作用。
Transmission of malaria depends upon the differentiation and development of the sexual stages of the parasite. In Plasmodium falciparum, it is a complex, multistage process, involving the expression of a large number of sexual stage-specific proteins. Pfg27 is one such protein, abundantly expressed at the onset of gametocytogenesis, We report successful disruption of the Pfg27 locus using homologous recombination and show that it is essential for the maintenance of the sexual phenotype. Transfectants lacking Pfg27 abort early in sexual development, resulting in vacuolated, highly disarranged, and disintegrating parasites. This suggests a critical role for Pfg27 in the sexual development of the parasite.