Osteogenic potential of cryopreserved human bone marrow-derived mesenchymal stem cells cultured with autologous serum
Osteogenic potential of cryopreserved human bone marrow-derived mesenchymal stem cells cultured with autologous serum
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DOI：
10.1097/scs.0b013e318163f2cc
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发表时间：
2008-05-01
影响因子：
0.9
通讯作者：
Uchinuma, Eiju
中科院分区：
文献类型：
--
作者：
Matsuo, Aoi;Yamazaki, Yasuharu;Uchinuma, Eiju
牙槽裂二次植骨是唇腭裂患者最重要的治疗方式之一。然而，对于儿童来说，获取足够量的骨比较困难，而且由于移植骨吸收和骨生长可能导致牙槽裂变形，往往需要重复手术，给患者带来沉重负担。如果能够储存人骨髓来源的间充质干细胞（MSC），也就是说，如果从患者自己的骨髓中采集的冷冻保存的自体间充质干细胞可以用患者自己的血清进行培养和扩增，并且可以解冻和培养以供日后移植，那么负担可能会减轻。本研究通过解冻并培养冻存3个月以上的MSC来制备混合型骨替代物。将混合型骨替代物植入裸鼠皮下。移植后6周和9周，取出骨移植物，通过碱性磷酸酶活性和茜素红S染色测定自体血清培养的细胞的成骨潜力，并与胎牛血清培养的细胞进行比较。自体血清培养的MSCs与胎牛血清培养的MSCs成骨潜力无显着差异。结果表明使用自体血清培养的间充质干细胞和细胞库进行人工骨移植的可能性。
Secondary bone grafting in the alveolar cleft is one of the most important therapeutic modalities for patients with cleft lip and palate. However, in children, harvesting a sufficient amount of bone is difficult, and repeated operations are often required because deformation of the alveolar cleft may occur because of the grafted bone absorption and bone growth, which imposes a heavy burden on the patients. The burden may be reduced if the banking of human bone marrow-derived mesenchymal stem cells (MSCs) could be made possible, that is, if cryopreserved autologous MSCs, those that have been harvested from the patient's own bone marrow, could be cultured and expanded with the patient's own serum and can be thawed and cultivated for grafting at a later date. In the current study, a hybrid-type bone substitute was prepared by thawing and cultivating MSCs that have been cryopreserved for more than 3 months. The hybrid-type bone substitute was implanted subcutaneously in nude mice. At 6 and 9 weeks after grafting, the bone graft was removed, and the osteogenic potential of the cells cultured with autologous serum, as determined by alkaline phosphatase activity and alizarin red S staining, was compared with those cultured with fetal bovine serum. There was no significant difference in the osteogenic potential between MSCs cultured with autologous serum and those cultured with fetal bovine serum. The results suggest the possibility of artificial bone grafting using MSCs cultured with autologous serum and the banking of the cells.