Translation termination in Arabidopsis thaliana:: characterisation of three versions of release factor 1
Translation termination in Arabidopsis thaliana:: characterisation of three versions of release factor 1
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DOI：
10.1016/j.gene.2004.06.053
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发表时间：
2004-10-27
期刊：
影响因子：
3.5
通讯作者：
Brown, C
中科院分区：
文献类型：
--
作者：
Chapman, B;Brown, C
翻译终止在所有真核生物中都是由两个释放因子eRF1和eRF3介导的。大多数生物体只有一个eRF1基因，然而，在拟南芥中发现了三个eRF1等基因。它们在编码区没有内含子，这可能表明它们是假基因。然而，这两个基因都得到了表达，并能够挽救酵母erf1突变体，这表明酵母erf1突变体在酵母中具有功能冗余。虽然通常是一个高度精确的过程，但翻译终止可以由信使RNA （mRNA）中的序列元件指示失败。有趣的是，在这三个同工基因之一（称为eRF1-1）的停止密码子后面有一个特征良好的读出元件。在使用双荧光素酶报告基因的体外实验中，该元件被证明能够诱导读透，但令人惊讶的是，使用完整的基因背景无法检测到读透。这些结果强调了植物基因组中基因的多样性和重复，但也强调了跨王国翻译过程的保守性。(C) 2004 Elsevier B.V.版权所有
Translation termination is mediated in all eukaryotes by the two release factors eRF1 and eRF3. Most organisms have a single eRF1 gene, however, three isogenes of eRF1 are found in Arabidopsis thaliana. They have no introns in the coding region which may indicate that some are pseudogenes. However, each was expressed and able to rescue a temperature sensitive eRF1-mutant of Saccharomyces cerevisiae indicating functional redundancy in A. thaliana. While normally a highly accurate process, translation termination can be directed to fail by sequence elements within an messenger RNA (mRNA). Interestingly, a well-characterised readthrough element follows the stop codon in one of these three isogenes (designated eRF1-1). This element was shown to be capable of inducing readthrough in an in vitro assay using a dual luciferase reporter, but surprisingly readthrough could not be detected using the complete gene context. The results highlight the diversity and duplication of genes within plant genomes, but also emphasize the conservation of the translation process across kingdoms. (C) 2004 Elsevier B.V. All rights reserved.