Impact of deficiency in CCR2 and CX3CR1 receptors on monocytes trafficking in herpes simplex virus encephalitis
Impact of deficiency in CCR2 and CX3CR1 receptors on monocytes trafficking in herpes simplex virus encephalitis
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DOI：
10.1099/vir.0.041046-0
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发表时间：
2012-06-01
影响因子：
3.8
通讯作者：
Boivin, Guy
中科院分区：
文献类型：
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作者：
Boivin, Nicolas;Menasria, Rafik;Boivin, Guy
在CCR2和CX3CR1受体缺陷的小鼠中，研究了在单纯疱疹病毒1型脑炎(HSV-1)过程中，驻留的小胶质细胞和外周血单核/巨噬细胞的渗透在先天免疫反应中的作用。CCR2(-/-)、CX3CR1(-/-)和C57BL/6野生型(WT)雄性小鼠鼻腔感染7x10(5)p.f.u。一种HSV-1临床毒株，监测脑炎和存活的迹象。另外，分别用RT-PCR和磁珠免疫分析法检测脑组织病毒DNA载量和细胞因子水平。通过血液和脑白细胞的荧光激活细胞分选来评估细胞反应。感染CX3CR1(-/-)小鼠的平均预期寿命显著低于WT小鼠(P
The role played by resident microglia and by the infiltration of peripheral monocytes/macrophages in the innate immune response during herpes simplex virus type 1 (HSV-1) encephalitis was evaluated in mice deficient for the CCR2 and CX3CR1 receptors. CCR2(-/-), CX3CR1(-/-) and C57BL/6 wild-type (WT) male mice were infected intranasally with 7x10(5) p.f.u. of an HSV-1 clinical strain and monitored for signs of encephalitis and survival. In addition, brain viral DNA load and cytokine levels were evaluated by RT-PCR and magnetic bead-based immunoassay, respectively. The cellular response was assessed by fluorescence-activated cell sorting of blood and brain leukocytes. Infected CX3CR1(-/-) mice had a significantly lower mean life expectancy than WT mice (P