Expression of Inflammatory Mediators in Induced Sputum: Comparative Study in Asthma and COPD
Expression of Inflammatory Mediators in Induced Sputum: Comparative Study in Asthma and COPD
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DOI：
10.1007/5584_2016_165
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发表时间：
2018-01-01
期刊：
影响因子：
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通讯作者：
Krenke, Rafal
中科院分区：
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作者：
Paplinska-Goryca, Magdalena;Nejman-Gryz, Patrycja;Krenke, Rafal
哮喘和COPD是最常见的阻塞性肺疾病，其特征在于下气道中的炎症，其导致气流受限。不同的炎症介质被认为在这些疾病中起关键作用。本研究在13名哮喘患者、12名COPD患者和13名对照受试者中进行。通过真实的时间PCR评估诱导痰细胞中IL-6、IL-13、CXCL 8、TSLP、IL-33、IL-25、IL-17、ECP、肥大细胞类胰蛋白酶、CCL 24和CCL 26的mRNA表达。我们发现CXCL 8与中性粒细胞百分比密切相关，但在COPD和哮喘患者中差异显著。与非特应性哮喘相比，特应性哮喘患者中IL-17的表达较低。COPD组巨噬细胞百分比与肥大细胞类胰蛋白酶和ECP表达呈负相关，哮喘组巨噬细胞百分比与CXCL 8表达呈负相关。ECP的表达与CAT测量的COPD症状的严重程度呈负相关。我们的结论是，哮喘和COPD表现出气道细胞因子谱的显着重叠。因此，这两种疾病之间的区分是困难的，因为基于一个单一的细胞因子，这表明在这些阻塞性肺疾病中共享一个共同的细胞因子网络的表型的共存。
Asthma and COPD are the most common obstructive lung diseases characterized by inflammation in the lower airways which contribute to airflow limitation. Different inflammatory mediators are thought to play a key role in these diseases. This study was conducted in 13 patients with asthma, 12 patients with COPD, and 13 control subjects. The expression of mRNA of IL-6, IL-13, CXCL8, TSLP, IL-33, IL-25, IL-17, ECP, mast cell tryptase, CCL24, and CCL26 was assessed in induced sputum cells by real time PCR. We found that CXCL8 was strongly related to the neutrophil percentage but differed significantly in COPD and asthma patients. The expression of IL-17 was lower in patients with atopic asthma compared to non-atopic asthma. The percentage of macrophages correlated negatively with the expression of mast cell tryptase and ECP in COPD, and with CXCL8 in asthma. The expression of ECP correlated negatively with the severity of COPD symptoms measured by CAT. We conclude that asthma and COPD demonstrate a significant overlap in the airway cytokine profile. Thus, differentiation between the two diseases is difficult as based on a single cytokine, which suggests the coexistence of phenotypes sharing a common cytokine network in these obstructive lung diseases.