A Resolution of Reported Discrepancies in the Characteristics of Platelet Glycoproteins IV (GPIV) and III b (GPIII b)

A Resolution of Reported Discrepancies in the Characteristics of Platelet Glycoproteins IV (GPIV) and III b (GPIII b)
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解决所报告的血小板糖蛋白 IV (GPIV) 和 III b (GPIII b) 特性差异

DOI:
10.1055/s-0038-1645694
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发表时间:
1990
影响因子:
6.7
通讯作者:
G. Jamieson
G. Jamieson
中科院分区:
医学2区
文献类型:
--
作者:
N. Yamamoto;C. Romeuf;N. Tandon;G. Jamieson

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概述糖蛋白IV(GPIV)和糖蛋白IIIb(GPIIIb)这两个术语被互换使用,文献中的报道表明,这种糖蛋白的相对分子质量不同地描述为88,000或97,000,交叉电泳法具有快速的阳极迁移率,在氨基酸分析中纯化的糖蛋白具有13或1个蛋氨酸残基。为了解决这些差异,我们评估了GPIV在全血小板和分离后的特性。这些研究表明,术语GPIV定义了一种抗蛋白酶的血小板表面糖蛋白,其Mr为88,330±2,240,在免疫学上与CD36分化抗原相同,CD36分化抗原在交叉免疫电泳法上以相对缓慢的阳离子迁移率迁移,每摩尔含有∼13个蛋氨酸残基。
Summary The terms glycoprotein IV (GPIV) and glycoprotein IIIb (GPIIIb) have been used interchangeably and reports in the literature have indicated this glycoprotein as having a molecular weight variously described as either 88,000 or 97,000, a fast anodal mobility on crossed electrophoresis and either 13 or <1 methionine residues on amino acid analysis of the purified glycoprotein. To resolve these discrepancies, we have evaluated the characteristics of GPIV both in whole platelets and after isolation. These studies have shown that the term GPIV defines a protease-resistant platelet surface glycoprotein with Mr 88,330 ± 2,240 which is immunologically identical with the CD36 differentiation antigen, which migrates with a relatively slow anodal mobility on crossed immunoelectrophoresis and which contains ∼13 methionine residues per mole.
单克隆抗人单核细胞抗体 OKM1 和 OKM5 具有独特的组织分布,包括与血管内皮的不同反应性。
DOI: --
发表时间: 1984
期刊: Journal of immunology (Baltimore, Md. : 1950)
影响因子: --
作者:
Knowles2nd,DM;Tolidjian,B;Marboe,C;D'Agati,V;Grimes,M;Chess,L
通讯作者: Chess,L
通过单克隆抗体鉴定正常和恶性的人类骨髓细胞和单核细胞。
DOI: --
发表时间: 1982
期刊: Journal of immunology (Baltimore, Md. : 1950)
影响因子: --
作者:
Bernstein,ID;Andrews,RG;Cohen,SF;McMaster,BE
通讯作者: McMaster,BE
DOI: 10.1172/jci112918
发表时间: 1987-04-01
影响因子: 15.9
作者:
ASCH, AS;BARNWELL, J;NACHMAN, RL
通讯作者: NACHMAN, RL