SIMULATION AND OPTIMIZATION OF PEPTIDE SEPARATION BY CAPILLARY ELECTROPHORESIS
SIMULATION AND OPTIMIZATION OF PEPTIDE SEPARATION BY CAPILLARY ELECTROPHORESIS
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DOI：
10.1016/0021-9673(94)80083-9
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发表时间：
1994-09-30
影响因子：
4.1
通讯作者：
POPPE, H
中科院分区：
文献类型：
--
作者：
CIFUENTES, A;POPPE, H
比较了文献中出现的用于预测肽迁移率的不同迁移率方程，得到了将肽的各个蛋白水解种类的迁移率与其组成联系起来的修正方程：mu = 1758 log(1 + 0.297q)/M(0.411)，其中mu是10(-9) m(2)/s.V中的电泳迁移率，q是种类的电荷和M其分子质量的积分值。此外，还开发了肽的一组 pK(a) 值的粗略估计。使用来自文献或实验的肽的真实电泳图，证明了该方程与用于通过毛细管区带电泳预测化合物分离的计算机程序的有用性。
The different mobility equations that have appeared in the literature for predicting peptide mobility were compared, A modified equation that relates the mobility of individual proteolytic species of a peptide to its composition has been obtained: mu = 1758 log(1 + 0.297q)/M(0.411), where mu is the electrophoretic mobility in 10(-9) m(2)/s.V, q is the integral value of the charge of the species and M its molecular mass. Also, a rough estimation of the set of pK(a) values for a peptide was developed. The usefulness of this equation together with a computer program for predicting separations of compounds by capillary zone electrophoresis is demonstrated, employing real electropherograms of peptides from the literature or from experiments.