Hypothalamic and amygdaloid corticotropin-releasing hormone (CRH) and CRH receptor-1 mRNA expression in the stress-hyporesponsive late pregnant and early lactating rat
Hypothalamic and amygdaloid corticotropin-releasing hormone (CRH) and CRH receptor-1 mRNA expression in the stress-hyporesponsive late pregnant and early lactating rat
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DOI：
10.1016/s0169-328x(01)00137-1
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发表时间：
2001-07-13
期刊：
影响因子：
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通讯作者：
Aguilera, G
中科院分区：
文献类型：
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作者：
da Costa, APC;Ma, XM;Aguilera, G
本研究调查了妊娠晚期和哺乳期（第 3 天）应激低反应期以及处女应激反应女性中促肾上腺皮质激素释放激素 (CRH) 及其受体 CRHR-1 和精氨酸加压素 (AVP) mRNA 的表达。原位杂交组织化学显示，室旁核（PVN）中的基础CRH mRNA在怀孕大鼠中减少，在哺乳期大鼠中增加（与处女对照相比），而仅在处女大鼠中在束缚应激后增加。所有组中的基础 PVN CRHR-1 mRNA 均显着增加，但在怀孕大鼠中达到较低水平。哺乳期大鼠小细胞 PVN 中的基础 AVP mRNA 较高，与 CRH mRNA 相比，所有组的应激后其水平均有所增加。在内侧视前区（MPOA）中，与未交配和怀孕的大鼠相比，哺乳期雌性大鼠的 CRH mRNA 水平较高，并且出乎意料的是，仅在未交配的大鼠中，它们在应激后显着下降。哺乳期大鼠杏仁核中央和内侧核中的 CRH mRNA 水平高于处女或怀孕大鼠，并且应激对两组均没有影响。这些数据表明这些应激低反应期：（1）不依赖于PVN中基础CRH mRNA表达； (2) 似乎具有完整的压力激活的 PVN 传入通路，如保留 CRHR-1 和 AVP 对压力的反应所示，但信息可能会以不同的方式处理； (3) 与 MPOA 的 CRH 介导途径的改变有关。 (C) 2001 年由 Elsevier Science B.V. 出版
This study investigated the expression of corticotropin releasing hormone (CRH) and its receptor CRHR-1, and arginine vasopressin (AVP) mRNAs during the stress hyporesponsive periods of late pregnancy and lactation (day-3) and in virgin stress-responsive females. In situ hybridization histochemistry showed that basal CRH mRNA in the paraventricular nucleus (PVN) decreased in pregnant and increased in lactating rats (compared with virgin controls), whereas it increased after restraint stress only in virgin rats. Basal PVN CRHR-1 mRNA increased markedly in all groups but reached lower levels in pregnant rats. Basal AVP mRNA in the parvocellular PVN was higher in lactating rats, and in contrast to CRH mRNA, it increased after stress in all groups. In medial preoptic area (MPOA) CRH mRNA levels were higher in lactating females compared with virgin and pregnant rats, and unexpectedly they decreased markedly after stress only in virgin rats. CRH mRNA levels in the central and medial nuclei of the amygdala were higher in lactating rats than in virgin or pregnant ones, and stress had no effect in either group. These data suggest that these stress hyporesponsive periods: (1)do not depend on basal CRH mRNA expression in the PVN; (2) appear to have intact stress-activated afferent pathways to the PVN, as shown by preservation of CRHR-1 and AVP responses to stress, but the information may be differently processed; (3) are associated with an alteration in a CRH mediated pathway from the MPOA. (C) 2001 Published by Elsevier Science B.V.