Characterization of a Lectin in Human Plasma Analogous to Bovine Conglutinin
Characterization of a Lectin in Human Plasma Analogous to Bovine Conglutinin
复制标题
人血浆中类似于牛凝集素的凝集素的表征
DOI：
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发表时间：
1987
影响因子：
3.7
通讯作者：
J. Jensenius
中科院分区：
文献类型：
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作者：
S. Thiel;G. Baatrup;P. Friis;S. Svehag;J. Jensenius
研究了一种类似于牛血浆蛋白的人血浆蛋白的结构特征。先前发现该蛋白质以钙依赖性和N-乙酰-D-葡萄糖胺可降解的方式与补体反应IgG结合，并进一步显示出与抗牛连接蛋白抗体的交叉反应性。通过凝胶渗透色谱法，人血浆中的伴发蛋白活性定位于含有约700，000的Mj蛋白质的级分。通过一种ELISA定位抗原决定簇，通过另一种ELISA定位生物活性。当在非还原条件下通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分析时，这些组分显示含有约300，000的蛋白质。当通过亲和色谱法部分纯化的人连接蛋白样蛋白通过SDS-PAGE和蛋白质印迹法进行未还原分析时，交叉反应的抗牛连接蛋白抗体与Mr为330，000的蛋白质结合。当样品在电泳前被还原和烷基化时，66，000的条带被免疫染色。330，000和66，000蛋白质被证明对胶原酶敏感。当与部分纯化的人连接蛋白的蛋白质印迹孵育时，观察到125 I-iC 3b与330，000条带结合。
The structural characteristics of a human plasma protein analogous to bovine conglutinin were studied. The protein was previously found to bind to complement‐reacted IgG in a calcium‐dependent and N‐acetyl‐D‐glucosamine‐inhibitable manner and it further shows cross‐reactivity with anti‐bovine conglutinin antibody. By gel permeation chromatography the conglutinin activity in human plasma was localized to fractions containing proteins of Mj at around 700,000. The conglutinin was localized by one ELISA for antigen determinants and by another for biological activity. When analysed by sodium dodecyl sulphate‐polyacrylamide gel electrophoresis (SDS‐PAGE) under non‐reducing conditions these fractions were shown to contain proteins of about 300,000. When human conglutinin‐like protein, partially purified by affinity chromatography, was analysed unreduced by SDS‐PAGE followed by western blotting, the cross‐reacting anti‐bovine conglutinin antibody bound to a protein with an Mr of 330,000. When the sample was reduced and alkylated before electrophoresis a band of 66,000 was immunostained. The 330,000 and 66,000 proteins were shown to be collagenase sensitive. 125I‐iC3b was seen to bind to the 330,000 band when incubated with western blots of partially purified human conglutinin.