Therapeutic efficacy of tumor-targeting Salmonella typhimurium A1-R on human colorectal cancer liver metastasis in orthotopic nude-mouse models.
Therapeutic efficacy of tumor-targeting Salmonella typhimurium A1-R on human colorectal cancer liver metastasis in orthotopic nude-mouse models.
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DOI：
10.18632/oncotarget.5187
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发表时间：
2015-10-13
期刊：
影响因子：
--
通讯作者：
Hoffman RM
中科院分区：
文献类型：
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作者：
Murakami T;Hiroshima Y;Zhao M;Zhang Y;Chishima T;Tanaka K;Bouvet M;Endo I;Hoffman RM
肝转移是结肠癌和其他癌症死亡的最常见原因。一般来说，肝转移是难以治疗的。本研究的目的是确定肿瘤靶向鼠伤寒沙门氏菌A1-R对原位小鼠模型肝转移的疗效。在本研究中使用表达红色荧光蛋白（RFP）的HT-29人结肠癌细胞。S.鼠伤寒杆菌A1-R以时间依赖性方式感染HT-29细胞，在体外抑制癌细胞增殖。S.在HT-29-RFP的皮下肿瘤小鼠模型中，鼠伤寒A1-R促进肿瘤坏死并抑制肿瘤生长。在原位小鼠模型中，S.鼠伤寒A1-R靶向肝转移瘤，并显着降低其生长。本研究的结果表明，未来的临床潜力的S。鼠伤寒A1-R靶向肝转移。
Liver metastasis is the most frequent cause of death from colon and other cancers. Generally, liver metastasis is recalcitrant to treatment. The aim of this study is to determine the efficacy of tumor-targeting Salmonella typhimurium A1-R on liver metastasis in orthotopic mouse models. HT-29 human colon cancer cells expressing red fluorescent protein (RFP) were used in the present study. S. typhimurium A1-R infected HT-29 cells in a time-dependent manner, inhibiting cancer-cell proliferation in vitro. S. typhimurium A1-R promoted tumor necrosis and inhibited tumor growth in a subcutaneous tumor mouse model of HT-29-RFP. In orthotopic mouse models, S. typhimurium A1-R targeted liver metastases and significantly reduced their growth. The results of this study demonstrate the future clinical potential of S. typhimurium A1-R targeting of liver metastasis.