Evaluation of peak overlap in migration-time distributions determined by organelle capillary electrophoresis: Type-II error analogy based on statistical-overlap theory
Evaluation of peak overlap in migration-time distributions determined by organelle capillary electrophoresis: Type-II error analogy based on statistical-overlap theory
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DOI：
10.1016/j.chroma.2009.07.001
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发表时间：
2009-08-28
影响因子：
4.1
通讯作者：
Arriaga, Edgar A.
中科院分区：
文献类型：
--
作者：
Davis, Joe M.;Arriaga, Edgar A.
细胞器通常通过毛细管电泳（CE）和激光诱导荧光检测分离。通常，假设在CE中观察到的峰来自单个细胞器，峰重叠的发生可以忽略不计。在此假设下，将CE划分为不同的区域，并计算每个区域的观测峰数，得到迁移时间和迁移率分布。本文建立了基于统计重叠理论（SOT）的判据来检验可忽略峰重叠的假设，并预测其有效性的条件。对于具有恒定峰密度的CE区域，峰的数量（即单个细胞器的强度分布）和观察到的峰（即最大值）由概率分布建模。对于较小的峰重叠，分布部分合并。它们的合并是通过类比假设检验的ii型误差来描述的。为峰重叠量制定了标准，在该标准下，观察到的峰的数量有85%或90%的概率位于单个细胞器峰数量的95%置信区间内。对于这个或更小的峰重叠量，观察到的峰的数量是一个很好的近似峰的数量。一个简单的程序是开发评估峰重叠，只需要确定峰标准偏差。峰所占区域的持续时间，以及该区域观测到的峰的数量。该程序可以独立应用于分区CE的每个区域。该程序适用于线粒体CE。(C) 2009 Elsevier B.V.版权所有
Organelles commonly are separated by capillary electrophoresis (CE) with laser-induced-fluorescence detection. Usually, it is assumed that peaks observed in the CE originate from single organelles, with negligible occurrence of peak overlap. Under this assumption, migration-time and mobility distributions are obtained by partitioning the CE into different regions and Counting the number of observed peaks in each region. In this paper, criteria based on statistical-overlap theory (SOT) are developed to test the assumption of negligible peak overlap and to predict conditions for its validity. For regions of the CE having constant peak density, the numbers of peaks (i.e., intensity profiles of single organelles) and observed peaks (i.e., maxima) are modeled by probability distributions. For minor peak overlap, the distributions partially merge. and their mergence is described by an analogy to the Type-II error of hypothesis testing. Criteria are developed for the amount of peak overlap, at which the number of observed peaks has an 85% or 90% probability of lying within the 95% confidence interval of the number of peaks of single organelles. For this or smaller amounts of peak overlap, the number of observed peaks is a good approximation to the number of peaks. A simple procedure is developed for evaluating peak overlap, requiring determination of only the peak standard deviation. the duration of the region Occupied by peaks, and the number of observed peaks in the region. The procedure can be applied independently to each region of the partitioned CE. The procedure is applied to a mitochondrial CE. (C) 2009 Elsevier B.V. All rights reserved.