BDNF, TNFα, HSP90, CFH, and IL-10 Serum Levels in Patients with Early or Late Onset Alzheimer's Disease or Mild Cognitive Impairment
BDNF, TNFα, HSP90, CFH, and IL-10 Serum Levels in Patients with Early or Late Onset Alzheimer's Disease or Mild Cognitive Impairment
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DOI：
10.3233/jad-130497
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发表时间：
2013-01-01
影响因子：
4
通讯作者：
Yilmazer, Selma
中科院分区：
文献类型：
--
作者：
Gezen-Ak, Duygu;Dursun, Erdinc;Yilmazer, Selma
识别早期检测的生物标志物已成为治疗和预防阿尔茨海默病（AD）越来越重要的方法。在这项研究中，我们研究了脑源性神经营养因子（BDNF）、补体因子H（CFH）、肿瘤坏死因子α (TNF α)、白细胞介素10 （IL-10）和热休克蛋白90 （Hsp90）在土耳其人群队列中作为AD血清生物标志物的潜力，因为它们被认为与AD相关。采用ELISA法比较早发性AD （EOAD，发病年龄< 65,n = 22）、晚发性AD （LOAD，发病年龄bbb65， n = 54）和轻度认知障碍（MCI, n = 30）患者血清BDNF、CFH、TNF α、IL-10和Hsp90水平与年龄匹配的健康对照（年龄< 65,n = 18和bbb65， n = 32）。与年龄匹配的健康对照组相比，EOAD和LOAD组血清BDNF水平显著降低，TNF - α水平显著升高。LOAD组和健康对照组血清TNF α和IL-10水平存在相关性。与对照组相比，LOAD和MCI患者的血清CFH水平显著降低。与对照组相比，EOAD、LOAD和MCI患者的血清Hsp90水平显著降低。蛋白质错误折叠、炎症反应和神经营养因子合成减少都被认为与AD型脑病理有关，我们的研究结果表明这些改变可能从血清样本中追踪。为了准确的早期诊断阿尔茨海默病，在大规模人群研究中确定多种生物标志物的改变概况是很重要的。
Identifying early-detection biomarkers have become an increasingly important approach in the treatment and prevention of Alzheimer's disease (AD). In this study, we investigated the potential of brain-derived neurotrophic factor (BDNF), complement factor H(CFH), tumor necrosis factor-alpha (TNF alpha), interleukin 10 (IL-10), and heat shock protein 90 (Hsp90) as serum biomarkers for AD in a cohort of the Turkish population because they have been suggested to be associated with AD. Serum BDNF, CFH, TNF alpha, IL-10, and Hsp90 levels in three groups of patients, early-onset AD (EOAD; age of onset < 65; n = 22), late-onset AD (LOAD; age of onset > 65; n = 54), and mild cognitive impairment (MCI) (n = 30), were compared with age-matched healthy controls (age < 65, n = 18 and age > 65; n = 32) using ELISA. The serum BDNF levels significantly decreased and TNF alpha levels significantly increased in the EOAD and LOAD groups compared to the age-matched healthy controls. There was a correlation between serum TNF alpha and IL-10 levels in the LOAD and healthy control groups. Serum CFH levels in the LOAD and MCI patients were significantly decreased compared with controls. Serum Hsp90 levels in the EOAD, LOAD, and MCI patients were significantly decreased compared with controls. The protein misfolding, the inflammatory response, and decreased neurotrophic factor synthesis are all suggested to be related to AD type brain pathology, and our results indicate these alterations might be traced from serum samples. For accurate early diagnosis of AD, it is important to determine a profile of alterations in multiple biomarkers in large-scale population studies.