Experimental study on dUTPase-inhibitor candidate and dUTPase/disaccharide mixtures by PCS and ENS
Experimental study on dUTPase-inhibitor candidate and dUTPase/disaccharide mixtures by PCS and ENS
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DOI：
10.1016/j.molstruc.2007.11.006
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发表时间：
2008-08-27
影响因子：
3.8
通讯作者：
Magazu, S.
中科院分区：
文献类型：
--
作者：
Varga, B.;Migliardo, F.;Magazu, S.
利用光子相关光谱（PCS）和弹性中子散射（ENS）研究了dUTPase-inhibitor candidate和dUTR/二糖混合物的结构和动力学性质，PCS的结果强调了dUTR在低二糖浓度下的流体动力学半径不发生变化，而在高二糖浓度下，Stokes-Einstein定律被打破。另一方面，ENS结果表明，水合dUTpase/二糖系统的局部均方位移线性依赖于二糖/水粘度，突出了蛋白质动力学与周围基质的动力学耦合。(C)2007 Elsevier B. V.保留所有权利。
The present work is focused on the structural and dynamical properties of dUTPase-inhibitor candidate and dUTPase/disaccharide mixtures investigated by Photon Correlation Spectroscopy (PCS) and Elastic Neutron Scattering (ENS).PCS findings emphasise that the dUTPase hydrodynamic radius in solution does not change at low disaccharide concentrations, while reveal, at high disaccharide concentration, a breakdown of the Stokes-Einstein law. On the other hand, the ENS findings show that the local mean square displacement of hydrated dUTpase/disaccharide systems is linearly dependent on the disaccharide/water viscosity, highlighting that the protein dynamics is coupled with that of the surrounding matrix. (C) 2007 Elsevier B.V. All rights reserved.