A new paradigm in the periodontal disease progression: Gingival connective tissue remodeling with simultaneous collagen degradation and fibers thickening
A new paradigm in the periodontal disease progression: Gingival connective tissue remodeling with simultaneous collagen degradation and fibers thickening
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DOI：
10.1016/j.tice.2008.07.001
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发表时间：
2009-02-01
期刊：
影响因子：
2.6
通讯作者：
Stach-Machado, D. R.
中科院分区：
文献类型：
--
作者：
Lorencini, M.;Silva, J. A. F.;Stach-Machado, D. R.
细菌性牙菌斑被认为是牙周病的主要原因，但疾病的进展也与宿主的炎症反应有关。最早受影响的组织是牙龈，但这种情况发生的具体机制仍不清楚。通常，胶原蛋白降解被认为是牙周病进展的主要标志，但牙龈组织中纤维的组织仍然是未知的。本研究的目的是探讨牙龈细胞外基质在结扎诱导的牙周病模型。微生物群的分析表明革兰氏阴性/革兰氏阳性微生物的比例逐渐增加。上皮基底膜旁网硬蛋白纤维的组织结构没有差异，而牙龈结缔组织中胶原纤维的排列受到显著影响。有炎症的动物呈现出胶原纤维占据的总面积减少35%。然而，这些纤维更厚，更密集。这些改变涉及I型、III型和VI型胶原，如通过免疫组织化学测定的。结果表明，牙龈细胞外基质在炎症过程中发生了明显的重组，表明牙周病进展的新模式：胶原降解和纤维增厚，同时。(C)2008爱思唯尔有限公司版权所有。
Bacterial dental plaque is considered to be the main cause of periodontal diseases, but progression of the disease is also related to the host inflammatory response. The earliest affected tissue is the gingiva, but the specific mechanisms involved in the onset of this condition remain unclear. Frequently, collagen degradation is pointed as the main marker of periodontal disease progression, but the Organization of the fibers in the gingival tissue is still unknown. The aim of the present study was to investigate the gingival extracellular matrix in a model of ligature-induced periodontal disease. Analysis of the microbiota indicated a progressive increase in the ratio of Gram-negative/Gram-positive microorganisms. There was no difference in the organization of reticulin fibers next to the epithelial basement membrane, whereas the arrangement of collagen fibers in the gingival connective tissue was significantly affected. Animals with inflammation presented a reduction of 35% in the total area occupied by collagen fibers. However, these fibers were thicker and more densely packed. These alterations involve type I, type III and type VI collagens as determined by immunohistochemistry. The results demonstrated the Occurrence of marked reorganization of the gingival extracellular matrix in response to the inflammatory process, indicating new paradigm in the periodontal disease progression: collagen degradation and fibers thickening, Simultaneously. (C) 2008 Elsevier Ltd. All rights reserved.