Genetic characterization of three Cuban Trichomonas vaginalis virus. Phylogeny of Totiviridae family
Genetic characterization of three Cuban Trichomonas vaginalis virus. Phylogeny of Totiviridae family
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DOI：
10.1016/j.meegid.2011.10.020
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发表时间：
2012-01-01
影响因子：
3.2
通讯作者：
Fernandez-Calienes, Ayme
中科院分区：
文献类型：
--
作者：
Fraga, Jorge;Rojas, Lazara;Fernandez-Calienes, Ayme
阴道毛滴虫可被称为T.迷走神经炎病毒（TVV）。这种病毒感染可能对滴虫的毒力和疾病的发病机制有重要意义。在这项研究中，我们鉴定和遗传特征的三个菌株的TVV分离自T。在古巴流浪预测的衣壳蛋白和RNA依赖的RNA聚合酶的3个新序列与已知的20个TVV序列一致，并与全病毒科的其他21个病毒进行了系统发育分析。系统发育树显示了四个不同的单系分支。一种对应于TVV，另一种对应于HIV病毒、利什曼病毒和布氏艾美耳球虫病毒，另一种对应于Totivirus属的病毒，最后一种对应于贾第虫病毒。急诊在系统发育树中，布鲁内蒂病毒被鉴定为利什曼病毒和利什曼病毒分离株之间的独立分类群，与利什曼病毒最密切相关。TVV是一个单系群，与全病毒科的其他病毒不同。这一结果表明，TVV可能被归类为一个单独的属，而不是贾第虫病毒。与贾第鞭毛虫病毒属和Totivirus属中的其他原生动物和真菌病毒相比，TVV似乎与利什曼病毒属中的原生动物病毒和真菌病毒更密切相关。在TVV中，可以在毛滴虫病毒簇内识别出四个主要组，这与先前提出的物种分类相对应。需要进一步研究更多的TVV株，特别是TVV3和4株，以确定毛滴虫病毒属之间的系统发育关系，特别是TVV2和3是否也构成一个明确界定的组。(C)2011 Elsevier B.V.保留所有权利。
Trichomonas vaginalis can be infected with double stranded RNA (dsRNA) viruses known as T. vaginalis virus (TVV). This viral infection may have important implications for trichomonal virulence and disease pathogenesis. In this study we identified and genetic characterized three strains of TVVs isolated from T. vaginalis in Cuba. The three new predicted sequences of capsid protein and RNA-dependent RNA polymerase amounted to the previously determined 20 TVV sequences and other 21 viruses of Totiviridae family were used for a phylogenetic analysis. Four distinct monophyletic clades are shown in a phylogenetic tree. One corresponds with TVVs, other with Victorivirus, Leishmaniavirus and Eimeria brunetti virus and, other with viruses of the genus Totivirus and the last with Giardiavirus. The E. brunetti virus is identified in the phylogenetic tree as independent taxon between Leishmaniavirus and Victorivirus isolates, most closely related to Victorivirus. TVV constitute a monophyletic cluster distinguishable from all other viruses in Totiviridae family. This result suggested that TVV may be grouped in a separated genus and not inside of Giardiavirus. TVVs appear to be more closely related to protozoan viruses in the genus Leishmaniavirus and to fungal viruses in the genus Victorivirus than to other protozoan and fungal viruses in Giardiavirus and Totivirus. Among TVVs, four main groups can be recognized within Trichomonasvirus cluster, which correspond with the previous species classification proposed. Further studies, with more TVV strains, especially TVV3 and 4 strains, are needed in order to determine the phylogenetic relationship among Trichomonasvirus genus and specifically if TVV2 and 3 each also constitute a well-delimited group. (C) 2011 Elsevier B.V. All rights reserved.