A search for evidence of viral infection in pancreases of newly diagnosed patients with IDDM
A search for evidence of viral infection in pancreases of newly diagnosed patients with IDDM
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DOI：
10.1007/s001250050642
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发表时间：
1997-01-01
期刊：
影响因子：
8.2
通讯作者：
Hilton, DA
中科院分区：
文献类型：
--
作者：
Foulis, AK;McGill, M;Hilton, DA
在福尔马林固定的石蜡包埋的尸检胰腺组织中，从最近死于胰岛素依赖型糖尿病的患者身上寻找病毒感染的证据的技术被开发出来。对47例DNA保存良好的胰腺进行EB病毒聚合酶链式反应和巨细胞病毒套式聚合酶链式反应分析。应用原位杂交技术对29例RNA保存良好的胰腺组织切片进行肠道病毒和EB病毒的检测。对75例胰腺进行了流行性腮腺炎病毒免疫组织化学分析。在所研究的任何糖尿病胰腺中都没有检测到这些病毒。对照研究表明，所采用的技术与尸检时所做的培养一样敏感。在9名死于心肌炎的婴儿中，胰腺可以进行研究；其中5例在胰岛中可见肠道病毒。在胰岛素依赖型糖尿病临床发病时，这项研究中包括的4种病毒中的任何一种都不太可能在胰腺发生急性或持续性感染。
Techniques were developed to look for evidence of viral infection in formalin-fixed paraffin-embedded autopsy pancreatic tissues from patients who had died of recent-onset insulin-dependent diabetes mellitus. DNA extracted from 47 pancreases in which good DNA preservation was confirmed was analysed by a polymerase chain reaction for Epstein-Barr virus and by a nested polymerase chain reaction for cytomegalovirus. Histological sections from 29 pancreases in which there was good RNA preservation were tested for the presence of enterovirus and Epstein-Barr virus using in situ hybridization techniques. Seventy-five pancreases were analysed immunohistochemically for the presence of mumps virus. None of these viruses could be detected in any of the diabetic pancreases studied. Control studies suggested that the techniques employed were as sensitive as culture done at the time of autopsy. Pancreas was available for study in 9 infants who had died of myocarditis; enterovirus was demonstrable in islets in 5 of these cases. An acute or persisting infection in the pancreas at the time of clinical onset of insulin-dependent diabetes by any of the 4 virus included in this study seems unlikely.