An organoselenium compound inhibits Staphylococcus aureus biofilms on hemodialysis catheters in vivo.
An organoselenium compound inhibits Staphylococcus aureus biofilms on hemodialysis catheters in vivo.
复制标题
有机硒化合物可抑制体内血液透析导管上的金黄色葡萄球菌生物膜。
DOI：
10.1128/aac.05680-11
复制
发表时间：
2012
影响因子：
4.9
通讯作者：
Hamood,AbdulN
中科院分区：
文献类型：
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作者：
Tran,PhatL;Lowry,Nathan;Campbell,Thomas;Reid,TedW;Webster,DanielR;Tobin,Eric;Aslani,Arash;Mosley,Thomas;Dertien,Janet;Colmer-Hamood,JaneA;Hamood,AbdulN
中心静脉导管（CVCs）被致病菌定植导致导管相关性血流感染（CRBSIs）。这些定植细菌形成高度耐抗生素的生物膜。金黄色葡萄球菌是crbsi中最常见的分离病原体之一。用抗菌剂浸渍CVC表面在降低crbsi发生率方面具有不同程度的有效性。我们最近发现有机硒共价附着在磁盘上作为抗生物膜剂抑制金黄色葡萄球菌生物膜的发育。在这项研究中，我们研究了有机硒涂层在血液透析导管（HDCs）上对体外和体内金黄色葡萄球菌生物膜的抑制能力。在静态或流动连续培养系统中，金黄色葡萄球菌在涂有硒氰酸二乙酸（SCAA）的高密度纤维素上均不能形成生物膜。SCAA包被也抑制了金黄色葡萄球菌在HDCsin上的生物膜的生长3天。SCAA包被稳定，对细胞培养物和动物无毒。这种用SCAA涂覆HDCs内外表面的新方法有可能预防由金黄色葡萄球菌引起的导管相关感染。
Colonization of central venous catheters (CVCs) by pathogenic bacteria leads to catheter-related bloodstream infections (CRBSIs). These colonizing bacteria form highly antibiotic-resistant biofilms. Staphylococcus aureus is one of the most frequently isolated pathogens in CRBSIs. Impregnating CVC surfaces with antimicrobial agents has various degrees of effectiveness in reducing the incidence of CRBSIs. We recently showed that organoselenium covalently attached to disks as an antibiofilm agent inhibited the development of S. aureus biofilms. In this study, we investigated the ability of an organoselenium coating on hemodialysis catheters (HDCs) to inhibit S. aureus biofilmsin vitroandin vivo. S. aureus failed to develop biofilms on HDCs coated with selenocyanatodiacetic acid (SCAA) in either static or flowthrough continuous-culture systems. The SCAA coating also inhibited the development of S. aureus biofilms on HDCsin vivofor 3 days. The SCAA coating was stable and nontoxic to cell culture or animals. This new method for coating the internal and external surfaces of HDCs with SCAA has the potential to prevent catheter-related infections due to S. aureus.