Bioinspired Fluorescent Dyes Based on a Conformationally Locked Chromophore of the Fluorescent Protein Kaede
Bioinspired Fluorescent Dyes Based on a Conformationally Locked Chromophore of the Fluorescent Protein Kaede
复制标题
DOI：
10.1002/ejoc.201500721
复制
发表时间：
2015-09-01
影响因子：
2.8
通讯作者：
Baranov, Mikhail S.
中科院分区：
文献类型：
--
作者：
Baleeva, Nadezhda S.;Myannik, Ksenia A.;Baranov, Mikhail S.
发现了一类基于荧光蛋白Kaede的发色团的构象锁定杂环核心的新型荧光染料。在Kaede发色团的亚苄基片段处引入单个构象锁导致荧光量子产率（λ Y）增加一个数量级，红移约为0.001。60 nm的发射光谱。在Kaede发色团的乙烯片段处施加第二锁定提供了在α Y中的进一步增加。锁定类似物在广泛的溶剂中表现出明亮和红移的发射，这使得它们成为广泛的荧光标记应用的良好候选者。
A novel class of fluorescent dyes based on the conformationally locked heterocyclic core of the chromophore of the fluorescent protein Kaede was discovered. Introduction of a single conformational lock at the benzylidene fragment of the Kaede chromophore resulted in an increase in the fluorescence quantum yield (FQY) by one order of magnitude and a redshift of ca. 60 nm in the emission spectrum. Imposing the second lock at the ethylene fragment of the Kaede chromophore provided a further increase in the FQY. Locked analogues demonstrated bright and redshifted emission in a broad range of solvents, which makes them good candidates for a wide spectrum of fluorescent-labeling applications.