Cultured human chromaffin cells grafted in spinal subarachnoid space relieves allodynia in a pain rat model.
Cultured human chromaffin cells grafted in spinal subarachnoid space relieves allodynia in a pain rat model.
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DOI：
10.4097/kjae.2011.60.5.357
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发表时间：
2011-05
影响因子：
2.9
通讯作者：
Lee SA
中科院分区：
文献类型：
--
作者：
Jeon Y;Baek WY;Chung SH;Shin N;Kim HR;Lee SA
将异种嗜铬细胞植入脊髓蛛网膜下腔可以在神经性疼痛模型中产生镇痛作用。然而，异种嗜铬细胞移植存在从动物到人类的病毒或细菌感染的潜在风险，包括由于朊病毒传播而导致的脑病。本研究的目的是探讨开发治疗性嗜铬细胞均质来源的可能性。在坐骨神经慢性收缩损伤引起的大鼠神经病理性疼痛模型中评估了人嗜铬细胞（HCC）的抗异常疼痛作用。将用藻酸盐-聚-L-赖氨酸-藻酸盐包裹的HCC鞘内植入大鼠（n = 10），而将空胶囊鞘内植入作为对照（n = 8）。测量烟碱刺激前后封装的 HCC 的去甲肾上腺素水平。然后我们用丙酮进行了行为测试（冷异常性疼痛）。此外，为了评估从 HCC 释放的阿片肽对减轻疼痛的潜在贡献，所有动物都注射了纳洛酮。与基础水平相比，尼古丁刺激后去甲肾上腺素的浓度显着增加。与接受空胶囊的大鼠相比，鞘内植入封装的 HCC 显着减少了冷异常性疼痛（P < 0.05）。注射纳洛酮15分钟后，HCC大鼠的冷异常性疼痛显着减轻（P < 0.05），而对照动物的冷异常性疼痛程度没有变化。从这些结果来看，HCC 似乎有可能作为移植物的镇痛来源，提供减轻疼痛的神经活性物质。
Implantation of xenogenic chromaffin cells into the spinal subarachnoid space can produce analgesia in neuropathic pain models. However, transplantation of xenogeneic chromaffin cell has a potential risk of viral or bacterial infections from animals to humans including encephalopathy due to prion transmission. The aim of this study was to investigate the possibility of developing a homogeneic source of therapeutic chromaffin cells. Anti-allodynic effects of human chromaffin cells (HCCs) were evaluated in a neuropathic pain model in rats induced by chronic constriction injury of the sciatic nerve. HCCs encapsulated with alginate-poly-L-lysine-alginate were intrathecally implanted into rats (n = 10), while empty capsules were intrathecally implanted as a control (n = 8). Levels of norepinephrine from encapsulated HCCs before and after nicotinic stimulation were measured. We then perfomed a behavior test (cold allodynia) with acetone. In addition, to assess the potential contribution to pain reduction of opioid peptides released from the HCCs, all animals were injected with naloxone. The concentration of norepinephrine after nicotine stimulation was significantly increased compared to basal levels. Intrathecal implantation of encapsulated HCCs, significantly reduced cold allodynia as compared to rats receiving empty capsules (P < 0.05). Fifteen minutes after the injection of naloxone, cold allodynia significantly decreased in rats with HCCs (P < 0.05), while the degree of cold allodynia in control animals was unaltered. From these results, it appears that HCCs have a possibility as an analgesic source for transplants delivering pain-reducing neuroactive substances.