Echinococcus granulosus calreticulin:: Molecular characterization and hydatid cyst localization
Echinococcus granulosus calreticulin:: Molecular characterization and hydatid cyst localization
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DOI：
10.1016/j.molimm.2007.08.022
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发表时间：
2008-03-01
影响因子：
3.6
通讯作者：
Galanti, Norbel
中科院分区：
文献类型：
--
作者：
Cabezon, Carolina;Cabrera, Gonzalo;Galanti, Norbel
包虫病是一种主要的人畜共患公共卫生问题，在许多国家造成巨大的经济损失，影响人类和牲畜物种。钙网蛋白（Calreticulin，CRT）是一种多功能、保守的钙结合蛋白分子伴侣，存在于除红细胞外的所有高等生物细胞中。在其他寄生虫物种中，CRT作为其与脊椎动物宿主关系的几个免疫学方面的关键调节剂出现。本文报道了两个编码E.颗粒状CRT（EgCRT）。连同NCBI数据库中的另一个部分序列，现在提出了完整的EgCRT cDNA序列。EgCRT mRNA在可育和不育的包虫囊胚层以及原卵囊中表达相同。在棘球蚴中，EgCRT是一种50 kDa的蛋白，表达于胚泡层和原胚泡，主要在核周和胞质区。(c)2007爱思唯尔有限公司保留所有权利。
Hydatidosis, whose etiological agent is the larval stage of the platyhelminth parasite Echinococcus granulosus, is a major zoonotic public health problem and causes great economic losses in many countries, affecting humans and livestock species. Calreticulin (CRT) is a multifunctional and conserved chaperone calcium-binding protein, present in every cell of higher organisms, except erythrocytes. In other parasites species, CRT emerges as a key modulator of several immunological aspects of their relationships with their vertebrate hosts. We report herein the cloning of two DNA segments coding for sequences of E. granulosus CRT (EgCRT). Together with another partial sequence available at the NCBI database, a complete EgCRT cDNA sequence is now proposed. EgCRT mRNA is equally expressed in fertile and infertile hydatid cysts germinal layers, as well as in protoscoleces. In hydatid cysts, EgCRT is a 50 kDa protein, expressed in germinal layer and protoscoleces, mainly in perinuclear and cytoplasmic zones. (c) 2007 Elsevier Ltd. All rights reserved.