CELLULAR IMMORTALITY - A LATE EVENT IN THE PROGRESSION OF HUMAN SQUAMOUS-CELL CARCINOMA OF THE HEAD AND NECK ASSOCIATED WITH P53 ALTERATION AND A HIGH-FREQUENCY OF ALLELE LOSS
CELLULAR IMMORTALITY - A LATE EVENT IN THE PROGRESSION OF HUMAN SQUAMOUS-CELL CARCINOMA OF THE HEAD AND NECK ASSOCIATED WITH P53 ALTERATION AND A HIGH-FREQUENCY OF ALLELE LOSS
复制标题
DOI：
10.1002/mc.2940130408
复制
发表时间：
1995-08-01
影响因子：
4.6
通讯作者：
PARKINSON, EK
中科院分区：
文献类型：
--
作者：
EDINGTON, KG;LOUGHRAN, OP;PARKINSON, EK
许多人类肿瘤含有变异细胞，与正常细胞不同，它们在体外具有无限的增殖潜力。然而，人们对这些永生细胞与体内人类癌症的相关性知之甚少。为了研究人类肿瘤系统的永生性，我们建立了头颈鳞状癌（SCC-HN）不同阶段的培养物。所有肿瘤培养物都被转化，因为它们在表面或悬浮培养物中表现出非常低的角化，并且部分或完全抵抗悬浮诱导的死亡。在癌前红斑培养物中未检测到永生变异，但其频率随着肿瘤进展而增加，表明永生是癌发生的晚期事件。一些晚期癌症仍然产生衰老培养物，但值得注意的是，所有复发肿瘤都是永生的。永生而非衰老的癌培养物与 p53 功能障碍和高频率的等位基因丢失相关，表明肿瘤抑制基因失活。这些结果表明，至少存在两类在表型和基因型上不同的人类 SCC-HN，并且给定肿瘤的病理阶段不一定表明其包含的细胞类型。 (C) 1995 Wiley-Liss, Inc.
Many human tumors contain variant cells that, unlike their normal counterparts, possess indefinite proliferative potential in vitro. However, little is known of the relevance of these immortal cells to human carcinomas in vivo. To investigate immortality in a human tumor system, we established cultures from different stages of head and neck squamous carcinoma (SCC-HN). All the neoplastic cultures were transformed because they showed very low cornification in surface or suspension culture and were partially or completely resistant to suspension-induced death. Immortal variants were not detected in premalignant erythroplakia cultures, but their frequency increased with tumor progression, indicating that immortality is a late event in carcinogenesis. Some late-stage carcinomas still produced senescent cultures, but, significantly, all recurrent tumors were immortal. Immortal but not senescent carcinoma cultures were associated With p53 dysfunction and a high frequency of allele loss, indicative of tumor suppressor gene inactivation. These results show that there are at least two classes of human SCC-HN that are phenotypically and genotypically distinct and that the pathological stage of a given tumor is not necessarily indicative of the kind of cells it contains. (C) 1995 Wiley-Liss, Inc.