Identification of microRNA/target gene in the dentate gyrus of 7‑day‑old mice following isoflurane exposure
Identification of microRNA/target gene in the dentate gyrus of 7‑day‑old mice following isoflurane exposure
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DOI：
10.55782/ane-2022-009
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Jian-Xin Liu
中科院分区：
文献类型：
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作者：
Bing-Nan Yang;Chu-Tong Zhang;Le-Fan Liu;Xiao-Lin Wu;Hai-Bo Hu;Yu Chen;Muneeb Iqbal;Yan-Bing Ma;Jin-Song Zhou;Xin-Li Xiao;Jian-Xin Liu
Studies on rodents and nonhuman primates suggest that exposure to anesthetics, particularly in the young brain, is associated with neuronal apoptosis as well as hippocampal-dependent cognitive dysfunction. Disruption of the development of dentate gyrus may play an important role in anesthetics-induced neurotoxicity. However, the anesthetics triggered molecular events in the dentate gyrus of the developing brain are poorly understood. By integrating two independent data sets obtained from miRNA-seq and mRNA-seq respectively, this study aims to profile the network of miRNA and potential target genes, as well as relevant events occurring in the dentate gyrus of isoflurane exposed 7-day-old mice. We found that a single four hours exposure to isoflurane yielded 1059 pairs of differently expressed miRNAs/target genes in the dentate gyrus. Gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis further indicates that dysregulated miRNAs/target genes have far-reaching effects on the cellular pathophysiological events, such as cell apoptosis, axon development, and synaptic transmission. Our results would greatly broaden our functional understanding of the role of miRNA/target gene in the context of anesthetics-induced neurotoxicity.
