Calibrated Bioresorbable Microspheres as an Embolic Agent: An Experimental Study in a Rabbit Renal Model.
Calibrated Bioresorbable Microspheres as an Embolic Agent: An Experimental Study in a Rabbit Renal Model.
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DOI：
10.1016/j.jvir.2015.01.014
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发表时间：
2015-12
期刊：
影响因子：
--
通讯作者：
Golzarian J
中科院分区：
文献类型：
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作者：
Weng L;Seelig D;Rostamzadeh P;Golzarian J
目的：评价新型生物可吸收微球(BRMS)的吸收时间、组织反应及肾动脉栓塞后血管再通情况。对20只成年兔肾下极行肾动脉栓塞术，栓塞剂为300~500μm。栓塞术后即刻(0d)处死2只兔。分别于术后第3、7、10、14、21、30天处死3只兔。通过组织学和血管造影术评价肾实质的病理变化、BRMS降解和血管再通。成功地进行了栓塞术，所有动物都存活了下来，没有出现并发症。所有接受栓塞者在第0天后均观察到梗死灶。第3天BRMS中度降解(评分=1.07±0.06)，第10天前有95%的BRMS被吸收，以后各时间点的BRMS材料残留较少。血管造影术从第3天开始观察到部分血管再通，而组织学上在第7天首次发现新的毛细血管形成。与BRMS相关的血管炎症包括早期时间点(第3天至第10天)的急性异嗜性浸润物；随着BRMS的吸收，这一问题得到解决。梗死区的炎症和纤维化与梗死灶的进展相一致。BRMS在体内是可生物吸收的，大多数BRMS在第10天之前就被吸收了，组织反应轻微。血管再通继发于BRMS的吸收。
To evaluate the time frame of resorption and tissue response of newly developed bioresorbable microspheres (BRMS) and vessel recanalization after renal embolization. Embolization of lower poles of kidneys of 20 adult rabbits was performed with BRMS (300–500 μm). Two rabbits were sacrificed immediately after embolization (day 0). Three rabbits were sacrificed after follow-up angiography at 3, 7, 10, 14, 21, and 30 days. The pathologic changes in the renal parenchyma, BRMS degradation, and vessel recanalization were evaluated histologically and angiographically. Embolization procedures were successfully performed, and all animals survived without complication. Infarcts were observed in all kidneys that received embolization harvested after day 0. Moderate degradation of BRMS (score = 1.07 ± 0.06) was observed by day 3. Of BRMS, 95% were resorbed before day 10 with scant BRMS materials remaining in the arteries at later time points. Partial vessel recanalization was observed by angiography starting on day 3, whereas new capillary formation was first identified histologically on day 7. Vascular inflammation associated with BRMS consisted of acute, heterophilic infiltrate at earlier time points (day 3 to day 10); this was resolved with the resorption of BRMS. Inflammation and fibrosis within infarcted regions were consistent with progression of infarction. BRMS were bioresorbable in vivo, and most BRMS were resorbed before day 10 with a mild tissue reaction. Vessel recanalization occurred secondary to the resorption of BRMS.