Effect of selenium on the expression of Fas/FasL in T lymphocyte anti colonic cancer cells
Effect of selenium on the expression of Fas/FasL in T lymphocyte anti colonic cancer cells
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发表时间：
2001
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通讯作者：
Zheng Minhua
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作者：
Zheng Minhua
目的观察硒对T淋巴细胞抗结肠癌细胞Fas/FasL表达的影响。方法以硒代半胱氨酸为影响因素，T淋巴细胞为效应细胞，人结肠癌细胞（LoVo细胞：人结肠腺癌）为靶细胞。MTT法和吖啶橙子染色法检测LoVo细胞凋亡，RT PCR和杂交技术检测硒作用前后T细胞和靶细胞表达Fas/FasL和TNF的水平。结果MTT和吖啶橙子染色显示硒可增强T淋巴细胞的抗肿瘤作用。（25.12±3.91）%，（46.17±3.68）%，（P0.05）]，并诱导LoVo细胞凋亡，其作用与对照组比较差异有显著性（P <0.05）。（18.6±4.1）%，（32.7±2.1）%，（P0.05）]，呈时间和剂量依赖性。靶细胞（LoVo细胞）和效应细胞表达FasL和Fas mRNA的水平增加。结论硒可增强T淋巴细胞抗肿瘤作用，其机制与Fas/FasL高表达有关。[
Objective To observe the effects of selenium on the expression of Fas/FasL in T lymphocyte anti colonic cancer cells. Methods Selenocysteine served as an influencing factor, T lymphocyte as an effector and human colonic tumor cell (LoVo cell: human colonic adenocarcinoma) as a target. MTT and acridine orange dying were used to measure LoVo cells apoptosis, and RT PCR and hybridization techniques used to identify the levels of Fas/FasL and TNF expressed by T cells or target cells after use of selenium or not. Results MTT and acridine orange dying showed that selenium enhanced T lymphocyte anti tumor function [(25.12±3.91)%,(46.17±3.68)% respectively,( P 0.05)],and induced LoVo cells apoptosis and this effect was [ (18.6±4.1)%, (32.7±2.1)% respectively,( P 0.05)] at the proper concentration (0.5～1.0 mg/L) in time and dose dependent manners. And the levels of FasL and Fas mRNA expressed by target cells (LoVo cell) and effectors were increased. Conclusion Selenium enhanced T lymphocyte anti tumor function, which was related to high levels of Fas/FasL expression. [