Plasma endotoxin core antibody concentration and linear growth are unrelated in rural Malawian children aged 2-5 years.
Plasma endotoxin core antibody concentration and linear growth are unrelated in rural Malawian children aged 2-5 years.
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DOI：
10.1186/s13104-015-1238-1
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发表时间：
2015-06-24
影响因子：
1.8
通讯作者：
Manary M
中科院分区：
文献类型：
--
作者：
Benzoni N;Korpe P;Thakwalakwa C;Maleta K;Stephenson K;Manary M;Manary M
环境性肠病是发展中国家与线性生长减少相关的上消化道亚临床炎症。通常研究者使用活组织检查或双重糖吸收试验来评估环境性肠病。这种测试是时间和资源密集型的，限制了它们作为筛查方法的效用。血清内毒素核心抗体（EndoCab）浓度是肠道炎症和完整性的潜在指标，因此可能有助于预测环境性肠病。我们分析了2 - 5岁马拉维农村儿童血清EndoCab水平与线性生长和乳果糖-甘露醇测定结果的相关性。这是一项对388名农村无症状马拉维儿童的观察性研究，这些儿童自出生以来至少每3个月进行一次人体测量。2011年6月和7月，进行了双糖渗透性试验，并采集血清样本进行EndoCab测定。使用Pearson相关性、Student t检验和多变量线性回归来比较In EndoCab浓度与采样时和3个月后的年龄别身高z评分（HAZ）。还对ln EndoCab与来自结合血清采样进行的双重糖渗透性试验的测量值进行了相同的分析。在血清采样前几个月具有人体测量数据的儿童亚组中，使用Pearson相关性来估计ln EndoCab与近期线性生长之间的关系。Ln EndoCa B浓度与测量时的HAZ（B = − 0.078，P = 0.14）和随后3个月HAZ的变化（B = − 0.018，P = 0.27）均不相关。EndoCa B浓度与乳果糖排泄率（%）（B <0.001，P = 0.98）和乳果糖：甘露醇比率（B = 0.021，P = 0.62）均无关。亚组分析也未显示EndoCab与近期生长之间存在任何显著相关性。EndoCab滴度与马拉维农村学龄前儿童的生长或肠道通透性测量无关。
Environmental enteropathy is subclinical inflammation of the upper gastrointestinal tract associated with reduced linear growth in developing countries. Usually investigators have used biopsy or a dual sugar absorption test to assess environmental enteropathy. Such tests are time and resource intensive, restricting their utility as screening methods. Serum endotoxin core antibody (EndoCab) concentration is a potential indicator of intestinal inflammation and integrity, and thus may be useful to predict environmental enteropathy. We analyzed the association of serum EndoCab levels versus linear growth and lactulose-mannitol assay results in 2–5 year old rural Malawian children. This was an observational study of 388 rural, asymptomatic Malawian children who had anthropometric measurements taken at least every 3 months since birth. In June and July 2011, dual sugar permeability tests were performed and serum samples were drawn for EndoCab assays. Pearson correlation, Student’s t test and multivariable linear regression were used to compare ln EndoCab concentrations with height-for-age z scores (HAZ) at time of sampling and 3 months later. Identical analysis was also performed for ln EndoCab versus measurements from dual sugar permeability testing performed in conjunction with serum sampling. In a subgroup of children with anthropometric data in the months prior to serum sampling, Pearson correlation was used to estimate the relationship between ln EndoCab and recent linear growth. Ln EndoCab concentrations were not correlated with HAZ at time of measurement (B = −0.078, P = 0.14) nor change in HAZ over the subsequent 3 months HAZ (B = −0.018, P = 0.27). EndoCab concentration was not associated with %lactulose excretion (B < 0.001, P = 0.98) nor the lactulose:mannitol ratio (B = 0.021, P = 0.62). Subgroup analysis also did not reveal any significant association between EndoCab and recent growth. EndoCab titers were not correlated with measurements of growth or intestinal permeability in rural pre-school aged Malawian children.