The genesis and evolution of H9N2 influenza viruses in poultry from southern china, 2000 to 2005
The genesis and evolution of H9N2 influenza viruses in poultry from southern china, 2000 to 2005
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DOI：
10.1128/jvi.00979-07
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发表时间：
2007-10-01
影响因子：
5.4
通讯作者：
Guan, Y.
中科院分区：
文献类型：
--
作者：
Xu, K. M.;Smith, G. J. D.;Guan, Y.
在过去的20年中，H9 N2流感病毒已在不同亚洲国家的陆地家禽中确立。我们以前的研究表明，鹌鹑携带越来越多样化的新型H9 N2病毒，包括鸡/北京/1/94（Ck/Bei-like）和鹌鹑/香港/G1/97（G1-like）病毒。然而，自1999年以来，H9 N2病毒在不同类型家禽中的起源和进化尚未得到系统的研究。在本研究中，H9 N2病毒分离自鸡，鸭，和其他次要家禽物种的特点，遗传和抗原性。我们的研究结果表明，Ck/Bei类H9 N2病毒已被引入中国南方的许多不同类型的家禽，包括鹌鹑，鹧鸪，石鸡，野鸡，珍珠鸡和家鸭，而G1类病毒通常在鹌鹑中检测到，在其他次要家禽物种中检测到的频率较低，而在鸡和鸭中未检测到。基因分析发现了35种H9 N2病毒基因型，其中包括14种以前未发现的新基因型。我们的研究结果还表明，双向种间传播存在于不同类型的家禽。我们的研究表明，多种病毒谱系的长期共循环（例如，H5 N1和H9 N2病毒）在不同类型的家禽中的感染促进了频繁的重配事件，这是该地区目前H9 N2和H5 N1流感病毒遗传多样性巨大的主要原因。这种情况有利于具有大流行潜力的流感病毒的出现。
H9N2 influenza viruses have become established in terrestrial poultry in different Asian countries over the last 2 decades. Our previous study demonstrated that quail harbor increasingly diverse novel H9N2 reassortants, including both Chicken/Beijing/1/94 (Ck/Bei-like) and Quail/Hong Kong/G1/97 (G1-like) viruses. However, since 1999, the genesis and evolution of H9N2 viruses in different types of poultry have not been investigated systematically. In the present study, H9N2 viruses isolated from chickens, ducks, and other minor poultry species were characterized genetically and antigenically. Our findings demonstrate that Ck/Bei-like H9N2 viruses have been introduced into many different types of poultry in southern China, including quail, partridges, chukar, pheasant, guinea fowl, and domestic ducks, while G1-like viruses were commonly detected in quail, less frequently detected in other minor poultry species, and not detected in chickens and ducks. Genetic analysis revealed 35 genotypes of H9N2 viruses, including 14 novel genotypes that have not been recognized before. Our results also suggested that two-way interspecies transmission exists between different types of poultry. Our study demonstrates that the long-term cocirculation of multiple virus lineages (e.g., H5N1 and H9N2 viruses) in different types of poultry has facilitated the frequent reassortment events that are mostly responsible for the current great genetic diversity in H9N2 and H5N1 influenza viruses in this region. This situation favors the emergence of influenza viruses with pandemic potential.