Immunohistochemical quantitation for extracellular matrix proteins in rats with glomerulonephritis induced by monoclonal anti-Thy-1.1 antibody.
Immunohistochemical quantitation for extracellular matrix proteins in rats with glomerulonephritis induced by monoclonal anti-Thy-1.1 antibody.
复制标题
单克隆抗 Thy-1.1 抗体诱导的肾小球肾炎大鼠细胞外基质蛋白的免疫组织化学定量。
作者：
N. Manabe;Y. Furuya;N. Nagano;M. Yagi;K. Kuramitsu;H. Miyamoto
细胞外基质蛋白（I型胶原和纤连蛋白）在肾皮质的冷冻组织切片与肾小球肾炎诱导单静脉注射抗Thy 1.1抗体的大鼠使用免疫组化微量法进行定量。实验性肾小球肾炎大鼠肾皮质I型胶原和纤维连接蛋白含量的变化（总蛋白分别为4.33 ± 0.79和10.41 ± 2.01微克/毫克）分别比给予正常小鼠IgG的对照大鼠高262%和151（分别为1.65 +/- 0.16和6.88 +/- 0.95微克/毫克;每种情况下p < 0.01）。在肾小球肾炎大鼠中，细胞外基质蛋白，特别是I型胶原的含量增加，与肾小球增加和系膜区扩张相关。I型胶原含量的增加与尿蛋白排泄量、血尿素氮和血清总胆固醇水平的增加密切相关（r分别为0.851、0.812和0.837;每种情况下p < 0.05）。肌酐清除率的降低与I型胶原含量的增加相关（r = 0.781; p < 0.05）。免疫组化显微方法可定量评价系膜增生性肾小球肾炎的组织病理学诊断。
Extracellular matrix proteins (type I collagen and fibronectin) in frozen histologic sections of kidney cortex from rats with glomerulonephritis induced by a single intravenous administration of anti-Thy 1.1 antibody were quantified using an immunohistochemical micromethod. Type I collagen and fibronectin contents in renal cortex of rats with experimental glomerulonephritis (4.33 +/- 0.79 and 10.41 +/- 2.01 microgram/mg of total protein, respectively) were 262% and 151%, respectively, higher than in control rats given normal mouse IgG (1.65 +/- 0.16 and 6.88 +/- 0.95 microgram/mg, respectively; p < 0.01 in each case). In the glomerulonephritic rats, the increase in the contents of extracellular matrix proteins, especially type I collagen, correlated with increasing glomeruli with expansion of mesangial areas. The increase in type I collagen content correlated well with increasing urinary protein excretion and blood urea nitrogen and serum total cholesterol levels (r = 0.851, 0.812, and 0.837, respectively; p < 0.05 in each case). The decrease in creatinine clearance correlated with increasing content of type I collagen (r = 0.781; p < 0.05). The immunohistochemical micromethod may make it possible to evaluate the histopathological diagnosis of mesangial proliferative glomerulonephritis quantitatively.