Timing in the regulation of neural crest cell migration: retarded "maturation" of regional extracellular matrix inhibits pigment cell migration in embryos of the white axolotl mutant.
Timing in the regulation of neural crest cell migration: retarded "maturation" of regional extracellular matrix inhibits pigment cell migration in embryos of the white axolotl mutant.
复制标题
神经嵴细胞迁移调节的时机：区域细胞外基质的延迟“成熟”抑制了白色蝾螈突变体胚胎中色素细胞的迁移。
DOI：
10.1016/s0012-1606(89)80048-x
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发表时间：
1989
影响因子：
2.7
通讯作者：
H. Epperlein
中科院分区：
文献类型：
--
作者：
J. Löfberg;R. Perris;H. Epperlein
在幼虫的白色蝾螈突变体（Ambystoma mexicanum），相反，正常的黑暗的，躯干色素沉着是有限的，因为表皮是无法支持色素细胞从神经嵴（NC）的表皮下迁移。本研究旨在探讨表皮下细胞外基质（ECM）是否是阻止白色胚胎色素细胞迁移的缺陷成分。我们移植表皮下ECM，在体内吸附在膜微载体，从和白色和黑暗的胚胎在各种组合。白色胚胎已显示出正常的NC细胞沿着中腹途径迁移，为了测试内侧ECM对表皮下迁移的影响，同样移植了该ECM。将带有ECM的载体在迁移前NC阶段皮下插入宿主胚胎中。不含ECM的对照载体和来自白色供体的含有表皮下ECM的载体不影响白色或深色胚胎中的NC细胞迁移。相比之下，来自深色供体的表皮下ECM在白色和深色宿主的表皮下空间中引发NC细胞迁移。值得注意的是，来自白色供体的表皮下ECM比通常使用的那些更老，也刺激了两种品系胚胎的迁移。同样地，来自白色供体的中间ECM在白色以及深色宿主中引起迁移。色素细胞出现在那些NC细胞中，这些NC细胞被刺激迁移，以响应与载体上的ECM接触。这些结果表明，白色胚胎的表皮下ECM作为色素细胞迁移的底物是暂时缺陷的，这意味着ECM的“成熟”被延迟超过色素细胞能够响应的时间。相反，白色胚胎的中间ECM似乎正常成熟。这些发现表明，d基因的作用是通过在有限的发展时期的表皮下ECM区域性表达。因此，d基因的作用似乎延缓了ECM的成熟，使其与色素细胞的迁移能力不同步。我们建议，这种转变的相对时间的发展现象涉及抑制色素细胞迁移的白色蝾螈突变体的胚胎，因此，限制色素的突变幼虫通过异时性产生。
In larvae of the white axolotl mutant (Ambystoma mexicanum), contrary to normal dark ones, trunk pigmentation is restricted because the epidermis is unable to support subepidermal migration of pigment cells from the neural crest (NC). This study examines whether the subepidermal extracellular matrix (ECM) is the defective component which prevents pigment cell migration in the white embryo. We transplanted subepidermal ECM, adsorbed in vivo on membrane microcarriers, from and to white and dark embryos in various combinations. White embryos have demonstrated normal NC cell migration along the medioventral pathway, and in order to test the effects of medial ECM on subepidermal migration, this ECM was similarly transplanted. Carriers with ECM attached were inserted subepidermally in host embryos at a premigratory NC stage. Control carriers without ECM and carriers with subepidermal ECM from white donors did not affect NC cell migration in white or dark embryos. In contrast, subepidermal ECM from dark donors triggered NC cell migration in the subepidermal space of both white and dark hosts. Remarkably, subepidermal ECM from white donors which were older than those normally used also stimulated migration in embryos of both strains. Likewise, medial ECM from white donors elicited migration in white as well as dark hosts. Pigment cells occurred among those NC cells that were stimulated to migrate in response to contact with ECM on carriers. These results indicate that the subepidermal ECM of the white embryo is transiently defective as a substrate for pigment cell migration, implying that “maturation” of the ECM is retarded beyond the times during which pigment cells are able to respond. In contrast, the medial ECM of the white embryo appears to mature normally. These findings suggest that the effect of thedgene is expressed regionally through the subepidermal ECM during a limited period of development. Hence, the action of thedgene seems to retard ECM maturation, bringing it out of phase with the migratory capability of the pigment cells. We propose that such a shift in relative timing of the developmental phenomena involved inhibits pigment cell migration in embryos of the white axolotl mutant and, accordingly, that the restricted pigmentation of the mutant larva is generated through heterochrony.
糖胺聚糖在无尾色素细胞迁移中的作用。
DOI：
--
发表时间：
1986
期刊：
Journal of embryology and experimental morphology
影响因子：
--
作者：
Tucker,RP
通讯作者：
Tucker,RP