Per and neuropeptide expression in the rat suprachiasmatic nuclei:: compartmentalization and differential cellular induction by light
Per and neuropeptide expression in the rat suprachiasmatic nuclei:: compartmentalization and differential cellular induction by light
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DOI：
10.1016/s0006-8993(02)03563-1
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发表时间：
2002-12-27
期刊：
影响因子：
2.9
通讯作者：
Masson-Pévet, M
中科院分区：
文献类型：
--
作者：
Dardente, H;Poirel, VJ;Masson-Pévet, M
Per 1和Per 2是两个在视交叉上核（SCN）中节律性表达的时钟基因。与昼夜节律起搏器的分子机制有关，并在光的夹带中起主要作用。迄今尚不清楚SCN（神经肽含量的异质结构）的每个细胞是否同样表达时钟基因。本研究的目的是确定，通过单和双非放射性和/或放射性杂交，细胞类型（AVP。VIP和GRP）在大鼠SCN中表达Per 1或Per 2，（1）当Per在白天高表达时。（2）夜间光脉冲诱导Per表达后。我们的研究结果表明，在白天，Per 1和Per 2基因都主要表达在AVP细胞的背正中部分的SCN，而只有少数VIP细胞的腹侧部分的SCN表现出Per基因的表达。相比之下，在夜间的光脉冲之后，存在两个Per基因的差异诱导。Per 1表达主要发生在腹外侧GRP细胞。而Per 2表达不限于SCN的视网膜受体部分，因为它也发生在AVP细胞中。总之，我们的研究结果表明，Per 1和Per 2主要表达在AVP细胞在白天，并表明GRP细胞在光的时钟重置中发挥重要作用。(C)2002 Elsevier Science B. V.保留所有权利。
Per1 and Per2, two clock genes rhythmically expressed in the suprachiasmatic nucleus (SCN). are implicated in the molecular mechanism of the circadian pacemaker and play a major role in its entrainment by light. To date. it is not known if every cell of the SCN, a heterogeneous structure in respect of neuropeptide content, expresses clock genes equally. The aim of this study was to identify, by single and double non-radioactive and/or radioactive hybridizations, the cell types (AVP. VIP and GRP) expressing Per1 or Per2 in the SCN of rats, (1) when Per are highly expressed during the daytime. and (2) after induction of Per expression by a light pulse at night. Our results indicate that, during the daytime, Per1 and Per2 genes are both mainly expressed in the AVP cells of the dorso-median part of the SCN, whereas only a few VIP cells in the ventral part of the SCN exhibit Per gene expression. In contrast, following a light pulse at night, there is differential induction of the two Per genes. Per1 expression essentially occurs in the ventro-lateral GRP cells. while Per2 expression is not restricted to the retinorecipient part of the SCN as it also occurs in AVP cells. Altogether, our results suggest that Per1 and Per2 are mainly expressed in AVP cells during the daytime and suggest that GRP cells play an important role in resetting of the clock by light. (C) 2002 Elsevier Science B.V. All rights reserved.