In vitro assays for cobblestone area-forming cells, LTC-IC, and CFU-C.
In vitro assays for cobblestone area-forming cells, LTC-IC, and CFU-C.
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DOI：
10.1007/978-1-59745-182-6_10
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发表时间：
2008
影响因子：
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通讯作者：
R. van Os;B. Dethmers-Ausema;G. de Haan
中科院分区：
文献类型：
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作者：
R. van Os;B. Dethmers-Ausema;G. de Haan
存在测量造血干细胞（HSC）的功能的各种测定。在本章中，描述了测量祖细胞（培养物中的集落形成单位; CFU-C）、干细胞（长期培养起始细胞; LTC-IC）或两者（鹅卵石区域形成细胞测定; CAFC）频率的体外测定。这些测定法回顾性地测量测试细胞群的潜力，即，当干细胞本身通常不再可检测时，其活性是明显的。尽管体外LTC-IC和CAFC试验已显示与体内活性相关，但体内移植试验（其中可显示细胞具有无限期地重新填充所有血液谱系的能力）是HSC活性的最终证据。然而，这些体外测定提供了筛选推定干细胞群体的干细胞活性或筛选某种治疗对HSC的作用的极好方法。
Various assays exist that measure the function of hematopoietic stemcells (HSCs). In this chapter, in vitro assays are described that measure the frequency of progenitors (colony-forming unit in culture; CFU-C), stem cells (long-term culture-initiating cell; LTC-IC), or both (cobblestone area-forming cell assay; CAFC). These assays measure the potential of a test cell population retrospectively, i.e., at the time its activity is evident when the stem cell itself is often not detectable anymore. Although the in vitro LTC-IC and CAFC assays have been shown to correlate with in vivo activity, in vivo transplantation assays, where it can be shown that cells possess the ability to indefinitely repopulate all blood lineages, are the ultimate proof for HSC activity. Nevertheless, these in vitro assays provide an excellent method to screen for stem cell activity of a putative stem cell population or for screening the effect of a certain treatment on HSCs.