Description of two novel members of the family Erysipelotrichaceae: Ileibacterium valens gen. nov., sp nov and Dubosiella newyorkensis, gen. nov., sp nov., from the murine intestine, and emendation to the description of Faecalibacterium rodentium
Description of two novel members of the family Erysipelotrichaceae: Ileibacterium valens gen. nov., sp nov and Dubosiella newyorkensis, gen. nov., sp nov., from the murine intestine, and emendation to the description of Faecalibacterium rodentium
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DOI：
10.1099/ijsem.0.001793
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发表时间：
2017-05-01
影响因子：
2.8
通讯作者：
Blaser, Martin J.
中科院分区：
文献类型：
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作者：
Cox, Laura M.;Sohn, Jiho;Blaser, Martin J.
为了更好地研究小鼠肠道微生物区系，从小鼠的小肠和大肠内容物中分离到大量革兰氏阳性菌(187株)，呈杆状和球状，兼性或严格厌氧。根据16S rRNA基因测序，共有115个分离物在系统发育上形成了三个不同的簇，位于白藜芦科。以菌株NYU-BL-A3(T)为代表的群1与杂色对虾的亲缘关系最近，16S rRNA基因序列相似性达87.7%。第二类群以NYU-BL-A4(T)为代表，与轮状巴氏杆菌亲缘关系最近，16S rRNA基因序列相似性达86.6%。第三组的16S rRNA基因序列(99.9%)与最近描述的也是从实验室小鼠身上发现的罗齿费卡利杆菌几乎相同；然而，这种菌株在生化特性上有一些不同，这一点在修订后的描述中有详细描述。NYU-BL-A3(T)株以C-16：0和C-18：0为主，NYU-BL-A4(T)株以C-10：0、C_1(6：0)、C_1(8：0)和C-18：1 omega 9c为主。这两个组还可以通过多个生化反应来区分，以NYU-BL-A4(T)为代表的组明显更活跃。根据系统发育、生化和化学分类标准，提出了两个新属：Valens Ilei杆菌gen。Nov.，sp.11月以NYU-BL-A3(T)(=ATCC TSD-63(T)=DSM 103668(T))为模式菌株，纽杜氏杜波氏杆菌为模式菌株。Nov.，sp.11月模式菌株为NYU-BL-A4(T)(=ATCC TSD-(T)=DSM 103457(T))。
To better characterize murine intestinal microbiota, a large number (187) of Gram-positive-staining, rod- and coccoid-shaped, and facultatively or strictly anaerobic bacteria were isolated from small and large intestinal contents from mice. Based on 16S rRNA gene sequencing, a total 115 isolates formed three phylogenetically distinct clusters located within the family Erysipelotrichaceae. Group 1, as represented by strain NYU-BL-A3(T), was most closely related to Allobaculum stercoricanis, with 16S rRNA gene sequence similarity values of 87.7 %. A second group, represented by NYU-BL-A4(T), was most closely related to Faecalibaculum rodentium, with 86.6% 16S rRNA gene sequence similarity. A third group had a nearly identical 16S rRNA gene sequence (99.9 %) compared with the recently described Faecalibaculum rodentium, also recovered from a laboratory mouse; however, this strain had a few differences in biochemical characteristics, which are detailed in an emended description. The predominant (> 10 %) cellular fatty acids of strain NYU-BL-A3(T) were C-16 : 0 and C-18 : 0, and those of strain NYU-BL-A4(T) were C-10 : 0, C1(6 : 0), C1(8 : 0) and C-18 : 1 omega 9c. The two groups could also be distinguished by multiple biochemical reactions, with the group represented by NYU-BL-A4(T) being considerably more active. Based on phyllogenetic, biochemical and chemotaxonomic criteria, two novel genera are proposed, Ileibacterium valens gen. nov., sp. nov. with NYU-BL-A3(T) (= ATCC TSD-63(T) = DSM 103668(T)) as the type strain and Dubosiella newyorkensis gen. nov., sp. nov. with NYU-BL-A4(T) (= ATCC TSD-64(T) = DSM 103457(T)) as the type strain.