TERTIARY MYOTUBES IN POSTNATAL GROWING PIG MUSCLE DETECTED BY THEIR MYOSIN ISOFORM COMPOSITION
TERTIARY MYOTUBES IN POSTNATAL GROWING PIG MUSCLE DETECTED BY THEIR MYOSIN ISOFORM COMPOSITION
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DOI：
10.2527/1992.7061806x
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发表时间：
1992-06-01
影响因子：
3.3
通讯作者：
BALLOCCHI, E
中科院分区：
文献类型：
--
作者：
MASCARELLO, F;STECCHINI, ML;BALLOCCHI, E
本试验对8 ~ 210日龄生长猪骨骼肌的生后发育进行了研究。猪半膜肌和背最长肌的冷冻切片的间接免疫过氧化物酶染色揭示了一个独特的人口的小纤维（三级肌管），特异性染色的抗体（抗NE）的选择性发育（胚胎和新生儿）亚型的肌肉肌球蛋白。在8日龄时，其他较大的纤维已经抗NE阴性，并分化为I型和II型。在猪的整个生长过程中，抗NE阳性纤维的数量逐渐减少，而剩余的阳性纤维的面积逐渐增加。这些结果可能表明，增生性生长不会在出生时停止。可能的机制来解释这些三级肌管含有肌球蛋白的发育亚型的起源提出了建议。
The postnatal development of skeletal muscles was studied in growing pigs from 8 to 210 d of age. Indirect immunoperoxidase staining of frozen sections of porcine semimembranosus muscle and longissimus muscle revealed a distinct population of small fibers (tertiary myotubes) that were stained specifically by an antibody (anti-NE) selective for the developmental (embryonic and neonatal) isoforms of muscle myosin. At 8 d of age the other larger fibers were already anti-NE negative and differentiated into Types I and II. A gradual decrease in the number of anti-NE positive fibers together with a gradual increase in area of the remaining positive fibers was observed throughout the pigs' growth. These results may indicate that hyperplastic growth does not cease at birth. Possible mechanisms to explain the origin of these tertiary myotubes containing developmental isoforms of myosin are suggested.